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Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil

One hundred and twenty-one isolates of endophytic fungi were recovered from leaves of the bioactive Brazilian plant species Ageratum myriadenia , Palicourea tetraphylla , Piptadenia adiantoides, and Trixis vauthieri. All fungal isolates were cultivated in liquid media and crude extracts were obtaine...

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Autores principales: Rosa, Luiz H., Gonçalves, Vívian N., Caligiorne, Rachel B., Alves, Tânia M. A., Rabello, Ana, Sales, Policarbo A., Romanha, Alvaro J., Sobral, Marcos E. G., Rosa, Carlos A., Zani, Carlos L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768680/
https://www.ncbi.nlm.nih.gov/pubmed/24031513
http://dx.doi.org/10.1590/S1517-838220100002000024
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author Rosa, Luiz H.
Gonçalves, Vívian N.
Caligiorne, Rachel B.
Alves, Tânia M. A.
Rabello, Ana
Sales, Policarbo A.
Romanha, Alvaro J.
Sobral, Marcos E. G.
Rosa, Carlos A.
Zani, Carlos L.
author_facet Rosa, Luiz H.
Gonçalves, Vívian N.
Caligiorne, Rachel B.
Alves, Tânia M. A.
Rabello, Ana
Sales, Policarbo A.
Romanha, Alvaro J.
Sobral, Marcos E. G.
Rosa, Carlos A.
Zani, Carlos L.
author_sort Rosa, Luiz H.
collection PubMed
description One hundred and twenty-one isolates of endophytic fungi were recovered from leaves of the bioactive Brazilian plant species Ageratum myriadenia , Palicourea tetraphylla , Piptadenia adiantoides, and Trixis vauthieri. All fungal isolates were cultivated in liquid media and crude extracts were obtained with ethyl acetate. The crude extracts were tested in bioassay panels using Leishmania amazonensis , Trypanosoma cruzi, the enzyme trypanothione reductase (TryR) from Trypanosoma cruzi, and three human cancer cell lines. Thirty-three extracts (27.2%) exhibited at least one biological activity. Seventeen extracts (14%) were cytotoxic against one or more human cancer cell line with the IC(50) values ranged of >0.2 to 25 µg/mL. Twenty-four extracts (19.8%) inhibited the activity of TryR, and three showed ability to inhibit the growth of T. cruzi above 60% and their IC(50) values ranged among 1 to 10 µg/mL. Eleven extracts (9%) were able to inhibit the growth of L. amazonensis and showed with IC(50) values ranged among 4.6 to 24.4 µg/mL. The endophytic fungi were identified as belonging to the genera Alternaria , Arthrinium , Cochliobolus , Colletotrichum , Penicillium , Fusarium, and Gibberella. An interesting result was obtained for the bioactive isolates UFMGCB 508, 537, 899 and 903, which were related to fungi associated with medicinal plants native to Asia, Australia, Africa, and Polynesia. These results indicate that bioactive plants living in Brazilian ecosystems are a potential host of endophytic fungi able to produce bioactive prototype molecules for drug development against neglected tropical diseases.
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spelling pubmed-37686802013-09-12 Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil Rosa, Luiz H. Gonçalves, Vívian N. Caligiorne, Rachel B. Alves, Tânia M. A. Rabello, Ana Sales, Policarbo A. Romanha, Alvaro J. Sobral, Marcos E. G. Rosa, Carlos A. Zani, Carlos L. Braz J Microbiol Environmental Microbiology One hundred and twenty-one isolates of endophytic fungi were recovered from leaves of the bioactive Brazilian plant species Ageratum myriadenia , Palicourea tetraphylla , Piptadenia adiantoides, and Trixis vauthieri. All fungal isolates were cultivated in liquid media and crude extracts were obtained with ethyl acetate. The crude extracts were tested in bioassay panels using Leishmania amazonensis , Trypanosoma cruzi, the enzyme trypanothione reductase (TryR) from Trypanosoma cruzi, and three human cancer cell lines. Thirty-three extracts (27.2%) exhibited at least one biological activity. Seventeen extracts (14%) were cytotoxic against one or more human cancer cell line with the IC(50) values ranged of >0.2 to 25 µg/mL. Twenty-four extracts (19.8%) inhibited the activity of TryR, and three showed ability to inhibit the growth of T. cruzi above 60% and their IC(50) values ranged among 1 to 10 µg/mL. Eleven extracts (9%) were able to inhibit the growth of L. amazonensis and showed with IC(50) values ranged among 4.6 to 24.4 µg/mL. The endophytic fungi were identified as belonging to the genera Alternaria , Arthrinium , Cochliobolus , Colletotrichum , Penicillium , Fusarium, and Gibberella. An interesting result was obtained for the bioactive isolates UFMGCB 508, 537, 899 and 903, which were related to fungi associated with medicinal plants native to Asia, Australia, Africa, and Polynesia. These results indicate that bioactive plants living in Brazilian ecosystems are a potential host of endophytic fungi able to produce bioactive prototype molecules for drug development against neglected tropical diseases. Sociedade Brasileira de Microbiologia 2010 2010-06-01 /pmc/articles/PMC3768680/ /pubmed/24031513 http://dx.doi.org/10.1590/S1517-838220100002000024 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Environmental Microbiology
Rosa, Luiz H.
Gonçalves, Vívian N.
Caligiorne, Rachel B.
Alves, Tânia M. A.
Rabello, Ana
Sales, Policarbo A.
Romanha, Alvaro J.
Sobral, Marcos E. G.
Rosa, Carlos A.
Zani, Carlos L.
Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil
title Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil
title_full Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil
title_fullStr Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil
title_full_unstemmed Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil
title_short Leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in Brazil
title_sort leishmanicidal, trypanocidal, and cytotoxic activities of endophytic fungi associated with bioactive plants in brazil
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768680/
https://www.ncbi.nlm.nih.gov/pubmed/24031513
http://dx.doi.org/10.1590/S1517-838220100002000024
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