Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR

Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovi...

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Detalles Bibliográficos
Autores principales: de Souza Figueiredo, Eduardo Eustáquio, Carvalho, Ricardo Cezar Tavares, Silvestre, Flávia Galindo, Lilenbaum, Walter, Fonseca, Leila Sousa, Silva, Joab Trajano, Paschoalin, Vânia Margaret Flosi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768681/
https://www.ncbi.nlm.nih.gov/pubmed/24031509
http://dx.doi.org/10.1590/S1517-838220100002000020
Descripción
Sumario:Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.

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