Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR

Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovi...

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Autores principales: de Souza Figueiredo, Eduardo Eustáquio, Carvalho, Ricardo Cezar Tavares, Silvestre, Flávia Galindo, Lilenbaum, Walter, Fonseca, Leila Sousa, Silva, Joab Trajano, Paschoalin, Vânia Margaret Flosi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768681/
https://www.ncbi.nlm.nih.gov/pubmed/24031509
http://dx.doi.org/10.1590/S1517-838220100002000020
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author de Souza Figueiredo, Eduardo Eustáquio
Carvalho, Ricardo Cezar Tavares
Silvestre, Flávia Galindo
Lilenbaum, Walter
Fonseca, Leila Sousa
Silva, Joab Trajano
Paschoalin, Vânia Margaret Flosi
author_facet de Souza Figueiredo, Eduardo Eustáquio
Carvalho, Ricardo Cezar Tavares
Silvestre, Flávia Galindo
Lilenbaum, Walter
Fonseca, Leila Sousa
Silva, Joab Trajano
Paschoalin, Vânia Margaret Flosi
author_sort de Souza Figueiredo, Eduardo Eustáquio
collection PubMed
description Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
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spelling pubmed-37686812013-09-12 Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR de Souza Figueiredo, Eduardo Eustáquio Carvalho, Ricardo Cezar Tavares Silvestre, Flávia Galindo Lilenbaum, Walter Fonseca, Leila Sousa Silva, Joab Trajano Paschoalin, Vânia Margaret Flosi Braz J Microbiol Veterinary Microbiology Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested. Sociedade Brasileira de Microbiologia 2010 2010-06-01 /pmc/articles/PMC3768681/ /pubmed/24031509 http://dx.doi.org/10.1590/S1517-838220100002000020 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
de Souza Figueiredo, Eduardo Eustáquio
Carvalho, Ricardo Cezar Tavares
Silvestre, Flávia Galindo
Lilenbaum, Walter
Fonseca, Leila Sousa
Silva, Joab Trajano
Paschoalin, Vânia Margaret Flosi
Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title_full Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title_fullStr Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title_full_unstemmed Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title_short Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title_sort detection of mycobacterium bovis dna in nasal swabs from tuberculous cattle by a multiplex pcr
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768681/
https://www.ncbi.nlm.nih.gov/pubmed/24031509
http://dx.doi.org/10.1590/S1517-838220100002000020
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