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Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates

The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production...

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Autores principales: de Oliveira, Caio Fernando, Salla, Adenilde, Lara, Valéria Maria, Rieger, Alexandre, Horta, Jorge André, Alves, Sydney Hartz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768689/
https://www.ncbi.nlm.nih.gov/pubmed/24031491
http://dx.doi.org/10.1590/S1517-83822010000200002
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author de Oliveira, Caio Fernando
Salla, Adenilde
Lara, Valéria Maria
Rieger, Alexandre
Horta, Jorge André
Alves, Sydney Hartz
author_facet de Oliveira, Caio Fernando
Salla, Adenilde
Lara, Valéria Maria
Rieger, Alexandre
Horta, Jorge André
Alves, Sydney Hartz
author_sort de Oliveira, Caio Fernando
collection PubMed
description The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1%) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8% of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR.
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spelling pubmed-37686892013-09-12 Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates de Oliveira, Caio Fernando Salla, Adenilde Lara, Valéria Maria Rieger, Alexandre Horta, Jorge André Alves, Sydney Hartz Braz J Microbiol Medical Microbiology The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1%) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8% of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR. Sociedade Brasileira de Microbiologia 2010 2010-06-01 /pmc/articles/PMC3768689/ /pubmed/24031491 http://dx.doi.org/10.1590/S1517-83822010000200002 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
de Oliveira, Caio Fernando
Salla, Adenilde
Lara, Valéria Maria
Rieger, Alexandre
Horta, Jorge André
Alves, Sydney Hartz
Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
title Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
title_full Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
title_fullStr Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
title_full_unstemmed Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
title_short Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
title_sort prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768689/
https://www.ncbi.nlm.nih.gov/pubmed/24031491
http://dx.doi.org/10.1590/S1517-83822010000200002
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