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Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates
The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768689/ https://www.ncbi.nlm.nih.gov/pubmed/24031491 http://dx.doi.org/10.1590/S1517-83822010000200002 |
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author | de Oliveira, Caio Fernando Salla, Adenilde Lara, Valéria Maria Rieger, Alexandre Horta, Jorge André Alves, Sydney Hartz |
author_facet | de Oliveira, Caio Fernando Salla, Adenilde Lara, Valéria Maria Rieger, Alexandre Horta, Jorge André Alves, Sydney Hartz |
author_sort | de Oliveira, Caio Fernando |
collection | PubMed |
description | The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1%) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8% of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR. |
format | Online Article Text |
id | pubmed-3768689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37686892013-09-12 Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates de Oliveira, Caio Fernando Salla, Adenilde Lara, Valéria Maria Rieger, Alexandre Horta, Jorge André Alves, Sydney Hartz Braz J Microbiol Medical Microbiology The emergence of Extended-Spectrum Beta-Lactamase (ESBL)-producing microorganisms in Brazilian hospitals is a challenge that concerns scientists, clinicians and healthcare institutions due to the serious risk they pose to confined patients. The goal of this study was the detection of ESBL production by clinical strains of Escherichia coli and Klebsiella sp. isolated from pus, urine and blood of patients at Hospital Universitário Santa Maria, Rio Grande Sul, RS, Brazil and the genotyping of the isolates based on bla SHV genes. The ESBL study was carried out using the Combined Disc Method, while Polymerase Chain Reaction (PCR) was used to study the bla SHV genes. Of the 90 tested isolates, 55 (61.1%) were identified as ESBL-producing by the combined disk method. The bla SHV genes were found in 67.8% of these microorganisms. K. pneumoniae predominated in the samples, presenting the highest frequency of positive results from the combined disk and PCR. Sociedade Brasileira de Microbiologia 2010 2010-06-01 /pmc/articles/PMC3768689/ /pubmed/24031491 http://dx.doi.org/10.1590/S1517-83822010000200002 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Medical Microbiology de Oliveira, Caio Fernando Salla, Adenilde Lara, Valéria Maria Rieger, Alexandre Horta, Jorge André Alves, Sydney Hartz Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates |
title | Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates |
title_full | Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates |
title_fullStr | Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates |
title_full_unstemmed | Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates |
title_short | Prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates |
title_sort | prevalence of extended-spectrum beta-lactamases-producing microorganisms in nosocomial patients and molecular characterization of the shv type isolates |
topic | Medical Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768689/ https://www.ncbi.nlm.nih.gov/pubmed/24031491 http://dx.doi.org/10.1590/S1517-83822010000200002 |
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