Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil

Many methods have been described for the detection of methicillin-resistant Staphylococcus aureus (MRSA), but the heterogeneous expression of methicillin resistance affects the reliability of these methods. The aim of the present study was to evaluate some methods for detecting methicillin resistanc...

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Autores principales: de Sousa Júnior, Francisco Canindé, Néri, Gildelane da Silva, Silva, Ana Karine, de Araújo, Bibiana Priscila Rodrigues Câmara, de Paiva Dourado Guerra, Myrian Júlia, de Britto Costa Fernandes, Maria José, Milan, Eveline Pipolo, de Melo, Maria Celeste Nunes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2010
Materias:
Medical Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768691/
https://www.ncbi.nlm.nih.gov/pubmed/24031498
http://dx.doi.org/10.1590/S1517-83822010000200009
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author de Sousa Júnior, Francisco Canindé
Néri, Gildelane da Silva
Silva, Ana Karine
de Araújo, Bibiana Priscila Rodrigues Câmara
de Paiva Dourado Guerra, Myrian Júlia
de Britto Costa Fernandes, Maria José
Milan, Eveline Pipolo
de Melo, Maria Celeste Nunes
author_facet de Sousa Júnior, Francisco Canindé
Néri, Gildelane da Silva
Silva, Ana Karine
de Araújo, Bibiana Priscila Rodrigues Câmara
de Paiva Dourado Guerra, Myrian Júlia
de Britto Costa Fernandes, Maria José
Milan, Eveline Pipolo
de Melo, Maria Celeste Nunes
author_sort de Sousa Júnior, Francisco Canindé
collection PubMed
description Many methods have been described for the detection of methicillin-resistant Staphylococcus aureus (MRSA), but the heterogeneous expression of methicillin resistance affects the reliability of these methods. The aim of the present study was to evaluate some methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil. Among the isolates, 15 were methicillin-susceptible and 45 were methicillin-resistant, including low-level heterogeneous resistance strains. Both the 30 ηg-cefoxitin disk and PBP2a test had 100% sensibility/specificity and appear to be good options for the detection of MRSA in the clinical laboratory.
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spelling pubmed-37686912013-09-12 Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil de Sousa Júnior, Francisco Canindé Néri, Gildelane da Silva Silva, Ana Karine de Araújo, Bibiana Priscila Rodrigues Câmara de Paiva Dourado Guerra, Myrian Júlia de Britto Costa Fernandes, Maria José Milan, Eveline Pipolo de Melo, Maria Celeste Nunes Braz J Microbiol Medical Microbiology Many methods have been described for the detection of methicillin-resistant Staphylococcus aureus (MRSA), but the heterogeneous expression of methicillin resistance affects the reliability of these methods. The aim of the present study was to evaluate some methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil. Among the isolates, 15 were methicillin-susceptible and 45 were methicillin-resistant, including low-level heterogeneous resistance strains. Both the 30 ηg-cefoxitin disk and PBP2a test had 100% sensibility/specificity and appear to be good options for the detection of MRSA in the clinical laboratory. Sociedade Brasileira de Microbiologia 2010 2010-06-01 /pmc/articles/PMC3768691/ /pubmed/24031498 http://dx.doi.org/10.1590/S1517-83822010000200009 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
de Sousa Júnior, Francisco Canindé
Néri, Gildelane da Silva
Silva, Ana Karine
de Araújo, Bibiana Priscila Rodrigues Câmara
de Paiva Dourado Guerra, Myrian Júlia
de Britto Costa Fernandes, Maria José
Milan, Eveline Pipolo
de Melo, Maria Celeste Nunes
Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil
title Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil
title_full Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil
title_fullStr Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil
title_full_unstemmed Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil
title_short Evaluation of different methods for detecting methicillin resistance in Staphylococcus aureus isolates in a university hospital located in the Northeast of Brazil
title_sort evaluation of different methods for detecting methicillin resistance in staphylococcus aureus isolates in a university hospital located in the northeast of brazil
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768691/
https://www.ncbi.nlm.nih.gov/pubmed/24031498
http://dx.doi.org/10.1590/S1517-83822010000200009
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