Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil

Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phyl...

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Detalles Bibliográficos
Autores principales: de Almeida Vaucher, Rodrigo, Dezen, Diogenes, Simonetti, Amauri Braga, Spilki, Fernando Rosado, Roehe, Paulo Michel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2011
Materias:
Veterinarian Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768747/
https://www.ncbi.nlm.nih.gov/pubmed/24031776
http://dx.doi.org/10.1590/S1517-838220110004000029
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author de Almeida Vaucher, Rodrigo
Dezen, Diogenes
Simonetti, Amauri Braga
Spilki, Fernando Rosado
Roehe, Paulo Michel
author_facet de Almeida Vaucher, Rodrigo
Dezen, Diogenes
Simonetti, Amauri Braga
Spilki, Fernando Rosado
Roehe, Paulo Michel
author_sort de Almeida Vaucher, Rodrigo
collection PubMed
description Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
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spelling pubmed-37687472013-09-12 Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil de Almeida Vaucher, Rodrigo Dezen, Diogenes Simonetti, Amauri Braga Spilki, Fernando Rosado Roehe, Paulo Michel Braz J Microbiol Veterinarian Microbiology Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3. Sociedade Brasileira de Microbiologia 2011 2011-12-01 /pmc/articles/PMC3768747/ /pubmed/24031776 http://dx.doi.org/10.1590/S1517-838220110004000029 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinarian Microbiology
de Almeida Vaucher, Rodrigo
Dezen, Diogenes
Simonetti, Amauri Braga
Spilki, Fernando Rosado
Roehe, Paulo Michel
Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_full Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_fullStr Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_full_unstemmed Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_short Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_sort phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from brazil
topic Veterinarian Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768747/
https://www.ncbi.nlm.nih.gov/pubmed/24031776
http://dx.doi.org/10.1590/S1517-838220110004000029
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