Stable expression and potential use of west nile virus envelope glycoproteins preM/E as antigen in diagnostic tests

West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acute...

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Autores principales: Cargnelutti, Juliana Felipetto, Brum, Mário Celso Sperotto, Weiblen, Rudi, Flores, Eduardo Furtado
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2011
Materias:
Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768760/
https://www.ncbi.nlm.nih.gov/pubmed/24031737
http://dx.doi.org/10.1590/S1517-838220110003000040
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author Cargnelutti, Juliana Felipetto
Brum, Mário Celso Sperotto
Weiblen, Rudi
Flores, Eduardo Furtado
author_facet Cargnelutti, Juliana Felipetto
Brum, Mário Celso Sperotto
Weiblen, Rudi
Flores, Eduardo Furtado
author_sort Cargnelutti, Juliana Felipetto
collection PubMed
description West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.
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spelling pubmed-37687602013-09-12 Stable expression and potential use of west nile virus envelope glycoproteins preM/E as antigen in diagnostic tests Cargnelutti, Juliana Felipetto Brum, Mário Celso Sperotto Weiblen, Rudi Flores, Eduardo Furtado Braz J Microbiol Veterinary Microbiology West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells. Sociedade Brasileira de Microbiologia 2011 2011-09-01 /pmc/articles/PMC3768760/ /pubmed/24031737 http://dx.doi.org/10.1590/S1517-838220110003000040 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
Cargnelutti, Juliana Felipetto
Brum, Mário Celso Sperotto
Weiblen, Rudi
Flores, Eduardo Furtado
Stable expression and potential use of west nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
title Stable expression and potential use of west nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
title_full Stable expression and potential use of west nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
title_fullStr Stable expression and potential use of west nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
title_full_unstemmed Stable expression and potential use of west nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
title_short Stable expression and potential use of west nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
title_sort stable expression and potential use of west nile virus envelope glycoproteins prem/e as antigen in diagnostic tests
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768760/
https://www.ncbi.nlm.nih.gov/pubmed/24031737
http://dx.doi.org/10.1590/S1517-838220110003000040
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