Cargando…

Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics

Several antihistaminics possess antibacterial activity against a broad spectrum of bacteria. However, the exact mechanism of such activity was unclear. Hence, the aim of this study is to investigate their mechanism of antibacterial activity especially their effect upon the permeability of the bacter...

Descripción completa

Detalles Bibliográficos
Autores principales: El-Nakeeb, Moustafa A., Abou-Shleib, Hamida M., Khalil, Amal M., Omar, Hoda G., El-Halfawy, Omar M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768765/
https://www.ncbi.nlm.nih.gov/pubmed/24031716
http://dx.doi.org/10.1590/S1517-838220110003000019
_version_ 1782283862495199232
author El-Nakeeb, Moustafa A.
Abou-Shleib, Hamida M.
Khalil, Amal M.
Omar, Hoda G.
El-Halfawy, Omar M.
author_facet El-Nakeeb, Moustafa A.
Abou-Shleib, Hamida M.
Khalil, Amal M.
Omar, Hoda G.
El-Halfawy, Omar M.
author_sort El-Nakeeb, Moustafa A.
collection PubMed
description Several antihistaminics possess antibacterial activity against a broad spectrum of bacteria. However, the exact mechanism of such activity was unclear. Hence, the aim of this study is to investigate their mechanism of antibacterial activity especially their effect upon the permeability of the bacterial cytoplasmic membrane. The effects of azelastine, cetirizine, cyproheptadine and diphenhydramine were studied using Gram-positive and Gram-negative multiresistant clinical isolates. Leakage of 260 and 280 nm UV-absorbing materials was detected upon treatment with the tested antihistaminics; indicative of membrane alteration. Using an artificial membrane model, cholesterol-free negatively-charged unilamellar liposomes, confirmed the effect of antihistaminics upon the membrane permeability both by showing an apparent membrane damage as observed microscopically and by detection of leakage of preloaded dye from the liposomes colorimatrically. Moreover, examination of the ultrastructure of cells treated with azelastine and cetirizine under the transmission electron microscope substantiated the detected abnormalities in the cell wall and membrane. Furthermore, the effect of pretreating certain isolates for both short and long periods with selected antihistaminics was followed by the viable count technique. Increased vulnerability towards further exposure to azelastine was observed in cells pretreated with azelastine for 2 days and those pretreated with azelastine or cetrizine for 30 days.
format Online
Article
Text
id pubmed-3768765
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37687652013-09-12 Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics El-Nakeeb, Moustafa A. Abou-Shleib, Hamida M. Khalil, Amal M. Omar, Hoda G. El-Halfawy, Omar M. Braz J Microbiol Medical Microbiology Several antihistaminics possess antibacterial activity against a broad spectrum of bacteria. However, the exact mechanism of such activity was unclear. Hence, the aim of this study is to investigate their mechanism of antibacterial activity especially their effect upon the permeability of the bacterial cytoplasmic membrane. The effects of azelastine, cetirizine, cyproheptadine and diphenhydramine were studied using Gram-positive and Gram-negative multiresistant clinical isolates. Leakage of 260 and 280 nm UV-absorbing materials was detected upon treatment with the tested antihistaminics; indicative of membrane alteration. Using an artificial membrane model, cholesterol-free negatively-charged unilamellar liposomes, confirmed the effect of antihistaminics upon the membrane permeability both by showing an apparent membrane damage as observed microscopically and by detection of leakage of preloaded dye from the liposomes colorimatrically. Moreover, examination of the ultrastructure of cells treated with azelastine and cetirizine under the transmission electron microscope substantiated the detected abnormalities in the cell wall and membrane. Furthermore, the effect of pretreating certain isolates for both short and long periods with selected antihistaminics was followed by the viable count technique. Increased vulnerability towards further exposure to azelastine was observed in cells pretreated with azelastine for 2 days and those pretreated with azelastine or cetrizine for 30 days. Sociedade Brasileira de Microbiologia 2011 2011-09-01 /pmc/articles/PMC3768765/ /pubmed/24031716 http://dx.doi.org/10.1590/S1517-838220110003000019 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
El-Nakeeb, Moustafa A.
Abou-Shleib, Hamida M.
Khalil, Amal M.
Omar, Hoda G.
El-Halfawy, Omar M.
Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics
title Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics
title_full Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics
title_fullStr Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics
title_full_unstemmed Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics
title_short Membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics
title_sort membrane permeability alteration of some bacterial clinical isolates by selected antihistaminics
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768765/
https://www.ncbi.nlm.nih.gov/pubmed/24031716
http://dx.doi.org/10.1590/S1517-838220110003000019
work_keys_str_mv AT elnakeebmoustafaa membranepermeabilityalterationofsomebacterialclinicalisolatesbyselectedantihistaminics
AT aboushleibhamidam membranepermeabilityalterationofsomebacterialclinicalisolatesbyselectedantihistaminics
AT khalilamalm membranepermeabilityalterationofsomebacterialclinicalisolatesbyselectedantihistaminics
AT omarhodag membranepermeabilityalterationofsomebacterialclinicalisolatesbyselectedantihistaminics
AT elhalfawyomarm membranepermeabilityalterationofsomebacterialclinicalisolatesbyselectedantihistaminics