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Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb
The lactic acid bacterium E isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16S rDNA fragment and species-specific PCR as Lactobacillus reuteri. Its potential antimicrobial activity and ability to modulate immune system in vitro and in vivo was determined. The grow...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768786/ https://www.ncbi.nlm.nih.gov/pubmed/24031741 http://dx.doi.org/10.1590/S1517-838220110003000044 |
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author | Sepová, H. Kiňová Dubnicková, M. Bilková, A. Bukovský, M. Bezáková, L. |
author_facet | Sepová, H. Kiňová Dubnicková, M. Bilková, A. Bukovský, M. Bezáková, L. |
author_sort | Sepová, H. Kiňová |
collection | PubMed |
description | The lactic acid bacterium E isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16S rDNA fragment and species-specific PCR as Lactobacillus reuteri. Its potential antimicrobial activity and ability to modulate immune system in vitro and in vivo was determined. The growth inhibition of potential pathogens decreased from Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica ser. Minnesota to Escherichia coli. The lowest inhibition activity was observed in the case of Candida albicans. The ability of L. reuteri E to modulate biological activities of human and mouse mononuclear cells was estimated in vitro and in vivo, respectively. The production of IL-1β by monocytes in vitro was significantly induced by L. reuteri E (relative activity 2.47). The ability to modulate biological activities of mononuclear cells by living L. reuteri E cells in vitro in comparison to disintegrated L. reuteri E cells in vivo differed. For example lysozyme activity in vitro was inhibited while in vivo was stimulated (relative activities 0.30 and 1.83, respectively). The peroxidase activity in vitro was stimulated while in vivo was inhibited (relative activities 1.53 and 0.17, respectively). Obtained results indicate that L. reuteri E is potential candidate to be used in probiotic preparations for animals and/or human. |
format | Online Article Text |
id | pubmed-3768786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37687862013-09-12 Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb Sepová, H. Kiňová Dubnicková, M. Bilková, A. Bukovský, M. Bezáková, L. Braz J Microbiol Microbial Physiology The lactic acid bacterium E isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16S rDNA fragment and species-specific PCR as Lactobacillus reuteri. Its potential antimicrobial activity and ability to modulate immune system in vitro and in vivo was determined. The growth inhibition of potential pathogens decreased from Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica ser. Minnesota to Escherichia coli. The lowest inhibition activity was observed in the case of Candida albicans. The ability of L. reuteri E to modulate biological activities of human and mouse mononuclear cells was estimated in vitro and in vivo, respectively. The production of IL-1β by monocytes in vitro was significantly induced by L. reuteri E (relative activity 2.47). The ability to modulate biological activities of mononuclear cells by living L. reuteri E cells in vitro in comparison to disintegrated L. reuteri E cells in vivo differed. For example lysozyme activity in vitro was inhibited while in vivo was stimulated (relative activities 0.30 and 1.83, respectively). The peroxidase activity in vitro was stimulated while in vivo was inhibited (relative activities 1.53 and 0.17, respectively). Obtained results indicate that L. reuteri E is potential candidate to be used in probiotic preparations for animals and/or human. Sociedade Brasileira de Microbiologia 2011 2011-09-01 /pmc/articles/PMC3768786/ /pubmed/24031741 http://dx.doi.org/10.1590/S1517-838220110003000044 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Microbial Physiology Sepová, H. Kiňová Dubnicková, M. Bilková, A. Bukovský, M. Bezáková, L. Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb |
title | Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb |
title_full | Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb |
title_fullStr | Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb |
title_full_unstemmed | Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb |
title_short | Identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb |
title_sort | identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb |
topic | Microbial Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768786/ https://www.ncbi.nlm.nih.gov/pubmed/24031741 http://dx.doi.org/10.1590/S1517-838220110003000044 |
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