Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China

Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 c...

Descripción completa

Detalles Bibliográficos
Autores principales: Huasai, Simujide, Chen, Aorigele, Wang, Chun-jie, Li, Yu, Tongrige, Bai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768809/
https://www.ncbi.nlm.nih.gov/pubmed/24031860
http://dx.doi.org/10.1590/S1517-83822012000200013
_version_ 1782283873169702912
author Huasai, Simujide
Chen, Aorigele
Wang, Chun-jie
Li, Yu
Tongrige, Bai
author_facet Huasai, Simujide
Chen, Aorigele
Wang, Chun-jie
Li, Yu
Tongrige, Bai
author_sort Huasai, Simujide
collection PubMed
description Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 cows. For categorization of E. coli, aEPEC was the category with widest distribution detected in 55 (18.15%) strains from 22 cattle. All of 84 PCR-positive strains belonged to 14 O serogroups, and O149 (25.00%) was most common identified, followed by O2 (17.86%), O8 (11.90%) and O103 (9.52%) with relatively high prevalence.
format Online
Article
Text
id pubmed-3768809
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37688092013-09-12 Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China Huasai, Simujide Chen, Aorigele Wang, Chun-jie Li, Yu Tongrige, Bai Braz J Microbiol Veterinary Microbiology Virulence genes of Escherichia coli (E. coli) isolates from healthy dairy cows were identified and characterized by a multiplex PCR assay and serogrouping test. The results showed that among the target genes, eaeA was most frequently detected, accounting for 22.11% (67/303) in all strains from 101 cows. For categorization of E. coli, aEPEC was the category with widest distribution detected in 55 (18.15%) strains from 22 cattle. All of 84 PCR-positive strains belonged to 14 O serogroups, and O149 (25.00%) was most common identified, followed by O2 (17.86%), O8 (11.90%) and O103 (9.52%) with relatively high prevalence. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768809/ /pubmed/24031860 http://dx.doi.org/10.1590/S1517-83822012000200013 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
Huasai, Simujide
Chen, Aorigele
Wang, Chun-jie
Li, Yu
Tongrige, Bai
Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_full Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_fullStr Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_full_unstemmed Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_short Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China
title_sort occurrence and characteristics of virulence genes of escherichia coli strains isolated from healthy dairy cows in inner mongolia, china
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768809/
https://www.ncbi.nlm.nih.gov/pubmed/24031860
http://dx.doi.org/10.1590/S1517-83822012000200013
work_keys_str_mv AT huasaisimujide occurrenceandcharacteristicsofvirulencegenesofescherichiacolistrainsisolatedfromhealthydairycowsininnermongoliachina
AT chenaorigele occurrenceandcharacteristicsofvirulencegenesofescherichiacolistrainsisolatedfromhealthydairycowsininnermongoliachina
AT wangchunjie occurrenceandcharacteristicsofvirulencegenesofescherichiacolistrainsisolatedfromhealthydairycowsininnermongoliachina
AT liyu occurrenceandcharacteristicsofvirulencegenesofescherichiacolistrainsisolatedfromhealthydairycowsininnermongoliachina
AT tongrigebai occurrenceandcharacteristicsofvirulencegenesofescherichiacolistrainsisolatedfromhealthydairycowsininnermongoliachina

Ejemplares similares