Evaluation of the Composition of Culture Medium for Yeast Biomass Production Using Raw Glycerol from Biodiesel Synthesis

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass a...

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Autores principales: dos Santos, Elisane Odriosolla, Michelon, Mariano, Furlong, Eliana Badiale, Burkert, Janaína Fernandes de Medeiros, Kalil, Susana Juliano, Burkert, Carlos André Veiga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Industrial Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768819/
https://www.ncbi.nlm.nih.gov/pubmed/24031849
http://dx.doi.org/10.1590/S1517-83822012000200002
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author dos Santos, Elisane Odriosolla
Michelon, Mariano
Furlong, Eliana Badiale
Burkert, Janaína Fernandes de Medeiros
Kalil, Susana Juliano
Burkert, Carlos André Veiga
author_facet dos Santos, Elisane Odriosolla
Michelon, Mariano
Furlong, Eliana Badiale
Burkert, Janaína Fernandes de Medeiros
Kalil, Susana Juliano
Burkert, Carlos André Veiga
author_sort dos Santos, Elisane Odriosolla
collection PubMed
description The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2(v)(5-1) fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).
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spelling pubmed-37688192013-09-12 Evaluation of the Composition of Culture Medium for Yeast Biomass Production Using Raw Glycerol from Biodiesel Synthesis dos Santos, Elisane Odriosolla Michelon, Mariano Furlong, Eliana Badiale Burkert, Janaína Fernandes de Medeiros Kalil, Susana Juliano Burkert, Carlos André Veiga Braz J Microbiol Industrial Microbiology The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2(v)(5-1) fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w). Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768819/ /pubmed/24031849 http://dx.doi.org/10.1590/S1517-83822012000200002 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Industrial Microbiology
dos Santos, Elisane Odriosolla
Michelon, Mariano
Furlong, Eliana Badiale
Burkert, Janaína Fernandes de Medeiros
Kalil, Susana Juliano
Burkert, Carlos André Veiga
Evaluation of the Composition of Culture Medium for Yeast Biomass Production Using Raw Glycerol from Biodiesel Synthesis
title Evaluation of the Composition of Culture Medium for Yeast Biomass Production Using Raw Glycerol from Biodiesel Synthesis
title_full Evaluation of the Composition of Culture Medium for Yeast Biomass Production Using Raw Glycerol from Biodiesel Synthesis
title_fullStr Evaluation of the Composition of Culture Medium for Yeast Biomass Production Using Raw Glycerol from Biodiesel Synthesis
title_full_unstemmed Evaluation of the Composition of Culture Medium for Yeast Biomass Production Using Raw Glycerol from Biodiesel Synthesis
title_short Evaluation of the Composition of Culture Medium for Yeast Biomass Production Using Raw Glycerol from Biodiesel Synthesis
title_sort evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768819/
https://www.ncbi.nlm.nih.gov/pubmed/24031849
http://dx.doi.org/10.1590/S1517-83822012000200002
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