Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method

Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensi...

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Autores principales: Monte, Leonardo Garcia, Jorge, Sérgio, Luiz, João Paulo Mesquita, Sinnott, Francine, Seixas, Kömmling Seixas, Aleixo, José Antonio Guimarães, Samartino, Luis Ernesto, Conceição, Fabricio Rochedo, Hartleben, Cláudia Pinho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768826/
https://www.ncbi.nlm.nih.gov/pubmed/24031870
http://dx.doi.org/10.1590/S1517-83822012000200023
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author Monte, Leonardo Garcia
Jorge, Sérgio
Luiz, João Paulo Mesquita
Sinnott, Francine
Seixas, Kömmling Seixas
Aleixo, José Antonio Guimarães
Samartino, Luis Ernesto
Conceição, Fabricio Rochedo
Hartleben, Cláudia Pinho
author_facet Monte, Leonardo Garcia
Jorge, Sérgio
Luiz, João Paulo Mesquita
Sinnott, Francine
Seixas, Kömmling Seixas
Aleixo, José Antonio Guimarães
Samartino, Luis Ernesto
Conceição, Fabricio Rochedo
Hartleben, Cláudia Pinho
author_sort Monte, Leonardo Garcia
collection PubMed
description Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis.
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spelling pubmed-37688262013-09-12 Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method Monte, Leonardo Garcia Jorge, Sérgio Luiz, João Paulo Mesquita Sinnott, Francine Seixas, Kömmling Seixas Aleixo, José Antonio Guimarães Samartino, Luis Ernesto Conceição, Fabricio Rochedo Hartleben, Cláudia Pinho Braz J Microbiol Veterinary Microbiology Diagnosis of leptospirosis by PCR is hampered due to the presence of substances on biological fluids. Here, we report an immunomagnetic separation step prior to PCR which improved the detection of Leptospira spp. in blood and urine samples from dogs. It resulted in a significant improvement on sensitivity for diagnosis of canine leptospirosis. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768826/ /pubmed/24031870 http://dx.doi.org/10.1590/S1517-83822012000200023 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
Monte, Leonardo Garcia
Jorge, Sérgio
Luiz, João Paulo Mesquita
Sinnott, Francine
Seixas, Kömmling Seixas
Aleixo, José Antonio Guimarães
Samartino, Luis Ernesto
Conceição, Fabricio Rochedo
Hartleben, Cláudia Pinho
Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title_full Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title_fullStr Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title_full_unstemmed Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title_short Diagnosis of canine leptospirosis using an immunomagnetic separation-PCR method
title_sort diagnosis of canine leptospirosis using an immunomagnetic separation-pcr method
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768826/
https://www.ncbi.nlm.nih.gov/pubmed/24031870
http://dx.doi.org/10.1590/S1517-83822012000200023
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