Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine

The aim of this study was to compare the PSPT standardized in-house as an alternative to MPT for potency assays of pertussis component. Statistical analyses have showed similar pertussis potency values when PSPT was compared to MPT. Significant correlation between the potency results obtained by in...

Descripción completa

Detalles Bibliográficos
Autores principales: Matos, Denise Cristina Souza, Marcovistz, Rugimar, da Silva, Andréa Marques Vieira, Quintilio, Wagner, Georgini, Ricardo Amaral
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Industrial Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768833/
https://www.ncbi.nlm.nih.gov/pubmed/24031848
http://dx.doi.org/10.1590/S1517-83822012000200001
_version_ 1782283878908559360
author Matos, Denise Cristina Souza
Marcovistz, Rugimar
da Silva, Andréa Marques Vieira
Quintilio, Wagner
Georgini, Ricardo Amaral
author_facet Matos, Denise Cristina Souza
Marcovistz, Rugimar
da Silva, Andréa Marques Vieira
Quintilio, Wagner
Georgini, Ricardo Amaral
author_sort Matos, Denise Cristina Souza
collection PubMed
description The aim of this study was to compare the PSPT standardized in-house as an alternative to MPT for potency assays of pertussis component. Statistical analyses have showed similar pertussis potency values when PSPT was compared to MPT. Significant correlation between the potency results obtained by in vivo and in vitro assays was also been observed. Results by PSPT have demonstrated reproducibility and accuracy for potency pertussis control and this approach has been considered promising for use at least during the steps of production.
format Online
Article
Text
id pubmed-3768833
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37688332013-09-12 Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine Matos, Denise Cristina Souza Marcovistz, Rugimar da Silva, Andréa Marques Vieira Quintilio, Wagner Georgini, Ricardo Amaral Braz J Microbiol Industrial Microbiology The aim of this study was to compare the PSPT standardized in-house as an alternative to MPT for potency assays of pertussis component. Statistical analyses have showed similar pertussis potency values when PSPT was compared to MPT. Significant correlation between the potency results obtained by in vivo and in vitro assays was also been observed. Results by PSPT have demonstrated reproducibility and accuracy for potency pertussis control and this approach has been considered promising for use at least during the steps of production. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768833/ /pubmed/24031848 http://dx.doi.org/10.1590/S1517-83822012000200001 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Industrial Microbiology
Matos, Denise Cristina Souza
Marcovistz, Rugimar
da Silva, Andréa Marques Vieira
Quintilio, Wagner
Georgini, Ricardo Amaral
Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title_full Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title_fullStr Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title_full_unstemmed Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title_short Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title_sort comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in dtp vaccine
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768833/
https://www.ncbi.nlm.nih.gov/pubmed/24031848
http://dx.doi.org/10.1590/S1517-83822012000200001
work_keys_str_mv AT matosdenisecristinasouza comparativeanalysisoftheintracerebralmouseprotectiontestandserologicalmethodforpotencyassaysofpertussiscomponentindtpvaccine
AT marcovistzrugimar comparativeanalysisoftheintracerebralmouseprotectiontestandserologicalmethodforpotencyassaysofpertussiscomponentindtpvaccine
AT dasilvaandreamarquesvieira comparativeanalysisoftheintracerebralmouseprotectiontestandserologicalmethodforpotencyassaysofpertussiscomponentindtpvaccine
AT quintiliowagner comparativeanalysisoftheintracerebralmouseprotectiontestandserologicalmethodforpotencyassaysofpertussiscomponentindtpvaccine
AT georginiricardoamaral comparativeanalysisoftheintracerebralmouseprotectiontestandserologicalmethodforpotencyassaysofpertussiscomponentindtpvaccine

Ejemplares similares