A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, th...

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Detalles Bibliográficos
Autores principales: Qiu, Shanlian, Chen, Jichen, Lin, Si, Lin, Xinjian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Environmental Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768845/
https://www.ncbi.nlm.nih.gov/pubmed/24031876
http://dx.doi.org/10.1590/S1517-83822012000200029
Descripción
Sumario:Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.

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