A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, th...

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Detalles Bibliográficos
Autores principales: Qiu, Shanlian, Chen, Jichen, Lin, Si, Lin, Xinjian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Environmental Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768845/
https://www.ncbi.nlm.nih.gov/pubmed/24031876
http://dx.doi.org/10.1590/S1517-83822012000200029
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author Qiu, Shanlian
Chen, Jichen
Lin, Si
Lin, Xinjian
author_facet Qiu, Shanlian
Chen, Jichen
Lin, Si
Lin, Xinjian
author_sort Qiu, Shanlian
collection PubMed
description Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
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spelling pubmed-37688452013-09-12 A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel Qiu, Shanlian Chen, Jichen Lin, Si Lin, Xinjian Braz J Microbiol Environmental Microbiology Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768845/ /pubmed/24031876 http://dx.doi.org/10.1590/S1517-83822012000200029 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Environmental Microbiology
Qiu, Shanlian
Chen, Jichen
Lin, Si
Lin, Xinjian
A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title_full A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title_fullStr A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title_full_unstemmed A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title_short A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel
title_sort comparison of silver staining protocols for detecting dna in polyester-backed polyacrylamide gel
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768845/
https://www.ncbi.nlm.nih.gov/pubmed/24031876
http://dx.doi.org/10.1590/S1517-83822012000200029
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