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In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China
Streptococcus suis is a swine pathogen and also a zoonotic agent. The formation of biofilms allows S. suis to become persistent colonizers and resist clearance by the host immune system and antibiotics. In this study, biofilm forming potentials of various S. suis strains were characterized by confoc...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768863/ https://www.ncbi.nlm.nih.gov/pubmed/24031918 http://dx.doi.org/10.1590/S1517-838220120003000021 |
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author | Dawei, Guo Liping, Wang Chengping, Lu |
author_facet | Dawei, Guo Liping, Wang Chengping, Lu |
author_sort | Dawei, Guo |
collection | PubMed |
description | Streptococcus suis is a swine pathogen and also a zoonotic agent. The formation of biofilms allows S. suis to become persistent colonizers and resist clearance by the host immune system and antibiotics. In this study, biofilm forming potentials of various S. suis strains were characterized by confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and tissue culture plates stained with crystal violet. In addition, the effects of five antimicrobial agents on biofilm formation were assayed in this study. S. suis produced biofilms on smooth and rough surface. The nutritional contents including glucose and NaCl in the growth medium modulated biofilm formation. There was a significant difference in their biofilm-forming ability among all 46 S. suis strains. The biofilm-forming potential of S. suis serotype 9 was stronger than type 2 and all other types. However, biofilm formation was inhibited by five commonly used antimicrobial agents, penicillin, erythromycin, azithromycin, ciprofloxacin, and ofloxacin at subinhibitory concentrations, among which inhibition of ciprofloxacin and ofloxacin was stronger than that of other three antimicrobial agents.Our study provides a detailed analysis of biofilm formation potential in S. suis, which is a step towards understanding its role in pathogenesis, and eventually lead to a better understanding of how to eradicate S. suis growing as biofilms with antibiotic therapy. |
format | Online Article Text |
id | pubmed-3768863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37688632013-09-12 In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China Dawei, Guo Liping, Wang Chengping, Lu Braz J Microbiol Veterinary Microbiology Streptococcus suis is a swine pathogen and also a zoonotic agent. The formation of biofilms allows S. suis to become persistent colonizers and resist clearance by the host immune system and antibiotics. In this study, biofilm forming potentials of various S. suis strains were characterized by confocal laser scanning microscopy (CLSM), scanning electron microscopy (SEM) and tissue culture plates stained with crystal violet. In addition, the effects of five antimicrobial agents on biofilm formation were assayed in this study. S. suis produced biofilms on smooth and rough surface. The nutritional contents including glucose and NaCl in the growth medium modulated biofilm formation. There was a significant difference in their biofilm-forming ability among all 46 S. suis strains. The biofilm-forming potential of S. suis serotype 9 was stronger than type 2 and all other types. However, biofilm formation was inhibited by five commonly used antimicrobial agents, penicillin, erythromycin, azithromycin, ciprofloxacin, and ofloxacin at subinhibitory concentrations, among which inhibition of ciprofloxacin and ofloxacin was stronger than that of other three antimicrobial agents.Our study provides a detailed analysis of biofilm formation potential in S. suis, which is a step towards understanding its role in pathogenesis, and eventually lead to a better understanding of how to eradicate S. suis growing as biofilms with antibiotic therapy. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768863/ /pubmed/24031918 http://dx.doi.org/10.1590/S1517-838220120003000021 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Veterinary Microbiology Dawei, Guo Liping, Wang Chengping, Lu In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China |
title | In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China |
title_full | In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China |
title_fullStr | In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China |
title_full_unstemmed | In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China |
title_short | In vitro biofilm forming potential of Streptococcus suis isolated from human and swine in China |
title_sort | in vitro biofilm forming potential of streptococcus suis isolated from human and swine in china |
topic | Veterinary Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768863/ https://www.ncbi.nlm.nih.gov/pubmed/24031918 http://dx.doi.org/10.1590/S1517-838220120003000021 |
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