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Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation

Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45°C on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces,...

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Autores principales: Moretti, Marcia M. S., Bocchini-Martins, Daniela A., Silva, Roberto Da, Rodrigues, André, Sette, Lara D., Gomes, Eleni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768882/
https://www.ncbi.nlm.nih.gov/pubmed/24031929
http://dx.doi.org/10.1590/S1517-838220120003000032
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author Moretti, Marcia M. S.
Bocchini-Martins, Daniela A.
Silva, Roberto Da
Rodrigues, André
Sette, Lara D.
Gomes, Eleni
author_facet Moretti, Marcia M. S.
Bocchini-Martins, Daniela A.
Silva, Roberto Da
Rodrigues, André
Sette, Lara D.
Gomes, Eleni
author_sort Moretti, Marcia M. S.
collection PubMed
description Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45°C on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from 40°C to 65°C and both endo-glucanase and xylanase from M. thermophila were stable in this temperature range when in absence of substrate. The enzymes were stable from pH 4.0 to 9.0.
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spelling pubmed-37688822013-09-12 Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation Moretti, Marcia M. S. Bocchini-Martins, Daniela A. Silva, Roberto Da Rodrigues, André Sette, Lara D. Gomes, Eleni Braz J Microbiol Industrial Microbiology Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45°C on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from 40°C to 65°C and both endo-glucanase and xylanase from M. thermophila were stable in this temperature range when in absence of substrate. The enzymes were stable from pH 4.0 to 9.0. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768882/ /pubmed/24031929 http://dx.doi.org/10.1590/S1517-838220120003000032 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Industrial Microbiology
Moretti, Marcia M. S.
Bocchini-Martins, Daniela A.
Silva, Roberto Da
Rodrigues, André
Sette, Lara D.
Gomes, Eleni
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
title Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
title_full Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
title_fullStr Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
title_full_unstemmed Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
title_short Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
title_sort selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768882/
https://www.ncbi.nlm.nih.gov/pubmed/24031929
http://dx.doi.org/10.1590/S1517-838220120003000032
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