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Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies
Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate t...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira de Microbiologia
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768885/ https://www.ncbi.nlm.nih.gov/pubmed/24031919 http://dx.doi.org/10.1590/S1517-838220120003000022 |
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author | Puentes, R Eliopulos, N Pérez, R Franco, G Sosa, K Bianchi, P Furtado, A Hübner, S.O. Esteves, P.A. |
author_facet | Puentes, R Eliopulos, N Pérez, R Franco, G Sosa, K Bianchi, P Furtado, A Hübner, S.O. Esteves, P.A. |
author_sort | Puentes, R |
collection | PubMed |
description | Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs. |
format | Online Article Text |
id | pubmed-3768885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37688852013-09-12 Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies Puentes, R Eliopulos, N Pérez, R Franco, G Sosa, K Bianchi, P Furtado, A Hübner, S.O. Esteves, P.A. Braz J Microbiol Veterinary Microbiology Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768885/ /pubmed/24031919 http://dx.doi.org/10.1590/S1517-838220120003000022 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Veterinary Microbiology Puentes, R Eliopulos, N Pérez, R Franco, G Sosa, K Bianchi, P Furtado, A Hübner, S.O. Esteves, P.A. Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies |
title | Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies |
title_full | Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies |
title_fullStr | Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies |
title_full_unstemmed | Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies |
title_short | Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies |
title_sort | isolation and characterization of canine parvovirus type 2c (cpv-2c) from symptomatic puppies |
topic | Veterinary Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768885/ https://www.ncbi.nlm.nih.gov/pubmed/24031919 http://dx.doi.org/10.1590/S1517-838220120003000022 |
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