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Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies

Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate t...

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Autores principales: Puentes, R, Eliopulos, N, Pérez, R, Franco, G, Sosa, K, Bianchi, P, Furtado, A, Hübner, S.O., Esteves, P.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768885/
https://www.ncbi.nlm.nih.gov/pubmed/24031919
http://dx.doi.org/10.1590/S1517-838220120003000022
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author Puentes, R
Eliopulos, N
Pérez, R
Franco, G
Sosa, K
Bianchi, P
Furtado, A
Hübner, S.O.
Esteves, P.A.
author_facet Puentes, R
Eliopulos, N
Pérez, R
Franco, G
Sosa, K
Bianchi, P
Furtado, A
Hübner, S.O.
Esteves, P.A.
author_sort Puentes, R
collection PubMed
description Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs.
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spelling pubmed-37688852013-09-12 Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies Puentes, R Eliopulos, N Pérez, R Franco, G Sosa, K Bianchi, P Furtado, A Hübner, S.O. Esteves, P.A. Braz J Microbiol Veterinary Microbiology Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells, demonstrated that CPV-2c is less citopathogenic in CRFK than in primary cultures. Our study represents the first report on isolation and characterization of canine parvovirus type 2c (CPV-2c) in cell cultures from South American dogs. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768885/ /pubmed/24031919 http://dx.doi.org/10.1590/S1517-838220120003000022 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
Puentes, R
Eliopulos, N
Pérez, R
Franco, G
Sosa, K
Bianchi, P
Furtado, A
Hübner, S.O.
Esteves, P.A.
Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies
title Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies
title_full Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies
title_fullStr Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies
title_full_unstemmed Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies
title_short Isolation and characterization of canine parvovirus type 2C (CPV-2C) from symptomatic puppies
title_sort isolation and characterization of canine parvovirus type 2c (cpv-2c) from symptomatic puppies
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768885/
https://www.ncbi.nlm.nih.gov/pubmed/24031919
http://dx.doi.org/10.1590/S1517-838220120003000022
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