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Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment

A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for dest...

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Detalles Bibliográficos
Autores principales: Oliveira, I.C., Almeida, R.C.C., Hofer, E., Almeida, P.F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768892/
https://www.ncbi.nlm.nih.gov/pubmed/24031937
http://dx.doi.org/10.1590/S1517-838220120003000040
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author Oliveira, I.C.
Almeida, R.C.C.
Hofer, E.
Almeida, P.F.
author_facet Oliveira, I.C.
Almeida, R.C.C.
Hofer, E.
Almeida, P.F.
author_sort Oliveira, I.C.
collection PubMed
description A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 10(8) pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an alternative for rapid, sensitive and quantitative detection of L. monocytogenes.
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spelling pubmed-37688922013-09-12 Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment Oliveira, I.C. Almeida, R.C.C. Hofer, E. Almeida, P.F. Braz J Microbiol Food Microbiology A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca. 10(8) pfu/mL) without affecting the viability of L. monocytogenes cells. Artificially inoculated skimmed milk revealed mean populations of the bacteria as low as between 13 cfu/mL (1.11 log cfu/mL), after a 10-h assay duration. Virucidal laser treatment demonstrated better protection of Listeria cells than the other agents previously tested. The protocol was faster and easier to perform than standard procedures. This protocol constitutes an alternative for rapid, sensitive and quantitative detection of L. monocytogenes. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768892/ /pubmed/24031937 http://dx.doi.org/10.1590/S1517-838220120003000040 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Food Microbiology
Oliveira, I.C.
Almeida, R.C.C.
Hofer, E.
Almeida, P.F.
Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
title Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
title_full Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
title_fullStr Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
title_full_unstemmed Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
title_short Bacteriophage amplification assay for detection of Listeria spp. using virucidal laser treatment
title_sort bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment
topic Food Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768892/
https://www.ncbi.nlm.nih.gov/pubmed/24031937
http://dx.doi.org/10.1590/S1517-838220120003000040
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