Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach

Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett–Burman design and four significant variables...

Descripción completa

Detalles Bibliográficos
Autores principales: Shabbiri, Khadija, Adnan, Ahmad, Jamil, Sania, Ahmad, Waqar, Noor, Bushra, Rafique, H.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Industrial Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768899/
https://www.ncbi.nlm.nih.gov/pubmed/24031928
http://dx.doi.org/10.1590/S1517-838220120003000031
Descripción
Sumario:Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett–Burman design and four significant variables (soybean meal, wheat bran, (NH(4))(2)SO(4) and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH(4))(2)SO(4)) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.

Ejemplares similares