Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach

Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett–Burman design and four significant variables...

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Detalles Bibliográficos
Autores principales: Shabbiri, Khadija, Adnan, Ahmad, Jamil, Sania, Ahmad, Waqar, Noor, Bushra, Rafique, H.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Industrial Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768899/
https://www.ncbi.nlm.nih.gov/pubmed/24031928
http://dx.doi.org/10.1590/S1517-838220120003000031
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author Shabbiri, Khadija
Adnan, Ahmad
Jamil, Sania
Ahmad, Waqar
Noor, Bushra
Rafique, H.M.
author_facet Shabbiri, Khadija
Adnan, Ahmad
Jamil, Sania
Ahmad, Waqar
Noor, Bushra
Rafique, H.M.
author_sort Shabbiri, Khadija
collection PubMed
description Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett–Burman design and four significant variables (soybean meal, wheat bran, (NH(4))(2)SO(4) and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH(4))(2)SO(4)) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.
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spelling pubmed-37688992013-09-12 Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach Shabbiri, Khadija Adnan, Ahmad Jamil, Sania Ahmad, Waqar Noor, Bushra Rafique, H.M. Braz J Microbiol Industrial Microbiology Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett–Burman design and four significant variables (soybean meal, wheat bran, (NH(4))(2)SO(4) and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH(4))(2)SO(4)) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768899/ /pubmed/24031928 http://dx.doi.org/10.1590/S1517-838220120003000031 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Industrial Microbiology
Shabbiri, Khadija
Adnan, Ahmad
Jamil, Sania
Ahmad, Waqar
Noor, Bushra
Rafique, H.M.
Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title_full Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title_fullStr Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title_full_unstemmed Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title_short Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
title_sort medium optimization of protease production by brevibacterium linens dsm 20158, using statistical approach
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768899/
https://www.ncbi.nlm.nih.gov/pubmed/24031928
http://dx.doi.org/10.1590/S1517-838220120003000031
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