Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China

This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin Medical University General Hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β...

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Autores principales: Li, Jing, Hu, Zhidong, Hu, Qiaojuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Medical Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768900/
https://www.ncbi.nlm.nih.gov/pubmed/24031907
http://dx.doi.org/10.1590/S1517-838220120003000010
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author Li, Jing
Hu, Zhidong
Hu, Qiaojuan
author_facet Li, Jing
Hu, Zhidong
Hu, Qiaojuan
author_sort Li, Jing
collection PubMed
description This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin Medical University General Hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs), respectively. Polymerase chain reactions and DNA sequencing confirmed that the strain carried IMP-4 metallo-β-lactamase (MBL) , SHV-11 and TEM-1 β-lactamase. Class I integron was positive and gave a 3.0-kb PCR amplicon .IMP-4 was located in Class I integron 5’CS. The gene determinants were organized in the order of bla(IMP-4)-orfII-orfIII.In all, the results show that IMP-4 MBL production caused the TJ8 resistance to carbapenems.
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spelling pubmed-37689002013-09-12 Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China Li, Jing Hu, Zhidong Hu, Qiaojuan Braz J Microbiol Medical Microbiology This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin Medical University General Hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs), respectively. Polymerase chain reactions and DNA sequencing confirmed that the strain carried IMP-4 metallo-β-lactamase (MBL) , SHV-11 and TEM-1 β-lactamase. Class I integron was positive and gave a 3.0-kb PCR amplicon .IMP-4 was located in Class I integron 5’CS. The gene determinants were organized in the order of bla(IMP-4)-orfII-orfIII.In all, the results show that IMP-4 MBL production caused the TJ8 resistance to carbapenems. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768900/ /pubmed/24031907 http://dx.doi.org/10.1590/S1517-838220120003000010 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
Li, Jing
Hu, Zhidong
Hu, Qiaojuan
Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China
title Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China
title_full Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China
title_fullStr Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China
title_full_unstemmed Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China
title_short Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China
title_sort isolation of the first imp-4 metallo-β-lactamase producing klebsiella pneumoniae in tianjin, china
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768900/
https://www.ncbi.nlm.nih.gov/pubmed/24031907
http://dx.doi.org/10.1590/S1517-838220120003000010
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AT huqiaojuan isolationofthefirstimp4metalloblactamaseproducingklebsiellapneumoniaeintianjinchina

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