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Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1–7 Isolated from bohai bay in china for detergent formulation

A novel alkaline lipase-producing strain 1–7 identified as Acinetobacter calcoaceticus was isolated from soil samples collected from Bohai Bay, China, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. The lipase from strain 1–7 showed the maximum activity at pH...

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Autores principales: Wang, Haikuan, Zhong, Shaojiong, Ma, Huijing, Zhang, Jie, Qi, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768965/
https://www.ncbi.nlm.nih.gov/pubmed/24031813
http://dx.doi.org/10.1590/S1517-838220120001000016
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author Wang, Haikuan
Zhong, Shaojiong
Ma, Huijing
Zhang, Jie
Qi, Wei
author_facet Wang, Haikuan
Zhong, Shaojiong
Ma, Huijing
Zhang, Jie
Qi, Wei
author_sort Wang, Haikuan
collection PubMed
description A novel alkaline lipase-producing strain 1–7 identified as Acinetobacter calcoaceticus was isolated from soil samples collected from Bohai Bay, China, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. The lipase from strain 1–7 showed the maximum activity at pH 9.0 under 40 °C. One interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. It is also stable at a broad range of pHs from 4.0 to 10.0 for 24 h. Its catalytic activity was highly enhanced in the presence of Ca(2+), Mg(2+) and K(+), but partially inhibited by Cu(2+), Al(3+), Fe(3+), Ba(2+)and Zn(2+). The fact that it displays marked stability and activity in the presence of TritonX-100, Tween-20, Tween-80, SDS, Hydrogen peroxide, Sodium perborate, Sodium hypochlorite, Sodium citrate, Sodium taurocholate, Glycerine and NaCl suggests that this lipase is suitable as an additive in detergent formulations.
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spelling pubmed-37689652013-09-12 Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1–7 Isolated from bohai bay in china for detergent formulation Wang, Haikuan Zhong, Shaojiong Ma, Huijing Zhang, Jie Qi, Wei Braz J Microbiol Industrial Microbiology A novel alkaline lipase-producing strain 1–7 identified as Acinetobacter calcoaceticus was isolated from soil samples collected from Bohai Bay, China, using an olive oil alkaline plate, which contained olive oil as the sole carbon source. The lipase from strain 1–7 showed the maximum activity at pH 9.0 under 40 °C. One interesting feature of this enzyme is that it exhibits lipase activity over a broad range of temperatures and good stability. It is also stable at a broad range of pHs from 4.0 to 10.0 for 24 h. Its catalytic activity was highly enhanced in the presence of Ca(2+), Mg(2+) and K(+), but partially inhibited by Cu(2+), Al(3+), Fe(3+), Ba(2+)and Zn(2+). The fact that it displays marked stability and activity in the presence of TritonX-100, Tween-20, Tween-80, SDS, Hydrogen peroxide, Sodium perborate, Sodium hypochlorite, Sodium citrate, Sodium taurocholate, Glycerine and NaCl suggests that this lipase is suitable as an additive in detergent formulations. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768965/ /pubmed/24031813 http://dx.doi.org/10.1590/S1517-838220120001000016 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Industrial Microbiology
Wang, Haikuan
Zhong, Shaojiong
Ma, Huijing
Zhang, Jie
Qi, Wei
Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1–7 Isolated from bohai bay in china for detergent formulation
title Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1–7 Isolated from bohai bay in china for detergent formulation
title_full Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1–7 Isolated from bohai bay in china for detergent formulation
title_fullStr Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1–7 Isolated from bohai bay in china for detergent formulation
title_full_unstemmed Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1–7 Isolated from bohai bay in china for detergent formulation
title_short Screening and characterization of a novel alkaline lipase from Acinetobacter calcoaceticus 1–7 Isolated from bohai bay in china for detergent formulation
title_sort screening and characterization of a novel alkaline lipase from acinetobacter calcoaceticus 1–7 isolated from bohai bay in china for detergent formulation
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768965/
https://www.ncbi.nlm.nih.gov/pubmed/24031813
http://dx.doi.org/10.1590/S1517-838220120001000016
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