16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting

Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the de...

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Autores principales: Navarro-Noya, Yendi, Hernández-Rodríguez, César, Zenteno, Juan C., Buentello-Volante, Beatriz, Cancino-Díaz, Mario E., Jan-Roblero, Janet, Cancino-Díaz, Juan C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Medical Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768976/
https://www.ncbi.nlm.nih.gov/pubmed/24031830
http://dx.doi.org/10.1590/S1517-838220120001000033
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author Navarro-Noya, Yendi
Hernández-Rodríguez, César
Zenteno, Juan C.
Buentello-Volante, Beatriz
Cancino-Díaz, Mario E.
Jan-Roblero, Janet
Cancino-Díaz, Juan C.
author_facet Navarro-Noya, Yendi
Hernández-Rodríguez, César
Zenteno, Juan C.
Buentello-Volante, Beatriz
Cancino-Díaz, Mario E.
Jan-Roblero, Janet
Cancino-Díaz, Juan C.
author_sort Navarro-Noya, Yendi
collection PubMed
description Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
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spelling pubmed-37689762013-09-12 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting Navarro-Noya, Yendi Hernández-Rodríguez, César Zenteno, Juan C. Buentello-Volante, Beatriz Cancino-Díaz, Mario E. Jan-Roblero, Janet Cancino-Díaz, Juan C. Braz J Microbiol Medical Microbiology Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768976/ /pubmed/24031830 http://dx.doi.org/10.1590/S1517-838220120001000033 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Medical Microbiology
Navarro-Noya, Yendi
Hernández-Rodríguez, César
Zenteno, Juan C.
Buentello-Volante, Beatriz
Cancino-Díaz, Mario E.
Jan-Roblero, Janet
Cancino-Díaz, Juan C.
16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title_full 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title_fullStr 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title_full_unstemmed 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title_short 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title_sort 16s rrna gene-based identification of bacteria in postoperative endophthalmitis by pcr-denaturing gradient gel electrophoresis (pcr-dgge) fingerprinting
topic Medical Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768976/
https://www.ncbi.nlm.nih.gov/pubmed/24031830
http://dx.doi.org/10.1590/S1517-838220120001000033
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