Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA

This study aimed to evaluate the use of the FTA elute card(TM) impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identi...

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Detalles Bibliográficos
Autores principales: Santos, Carla R., Franciscatto, Laura G., Barcellos, Regina B., Almeida, Sabrina E. M., Rossetti, Maria Lucia R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Genetics and Molecular Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768978/
https://www.ncbi.nlm.nih.gov/pubmed/24031844
http://dx.doi.org/10.1590/S1517-838220120001000047
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author Santos, Carla R.
Franciscatto, Laura G.
Barcellos, Regina B.
Almeida, Sabrina E. M.
Rossetti, Maria Lucia R.
author_facet Santos, Carla R.
Franciscatto, Laura G.
Barcellos, Regina B.
Almeida, Sabrina E. M.
Rossetti, Maria Lucia R.
author_sort Santos, Carla R.
collection PubMed
description This study aimed to evaluate the use of the FTA elute card(TM) impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples.
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spelling pubmed-37689782013-09-12 Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA Santos, Carla R. Franciscatto, Laura G. Barcellos, Regina B. Almeida, Sabrina E. M. Rossetti, Maria Lucia R. Braz J Microbiol Genetics and Molecular Microbiology This study aimed to evaluate the use of the FTA elute card(TM) impregnated with cervicovaginal sample directly in the PCR amplification for detection of HPV-DNA. The results were compared to a reference technique. This method was more efficient than the protocol indicated by the manufacturer, identifying 91.7% against 54.2% of the positive samples. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768978/ /pubmed/24031844 http://dx.doi.org/10.1590/S1517-838220120001000047 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Genetics and Molecular Microbiology
Santos, Carla R.
Franciscatto, Laura G.
Barcellos, Regina B.
Almeida, Sabrina E. M.
Rossetti, Maria Lucia R.
Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA
title Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA
title_full Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA
title_fullStr Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA
title_full_unstemmed Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA
title_short Use of FTA elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus DNA
title_sort use of fta elute card impregnated with cervicovaginal sample directly into the amplification reaction increases the detection of human papillomavirus dna
topic Genetics and Molecular Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768978/
https://www.ncbi.nlm.nih.gov/pubmed/24031844
http://dx.doi.org/10.1590/S1517-838220120001000047
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