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Production of Lytic Enzymes by Trichoderma Isolates during in vitro Antagonism with Aspergillus Niger, The Causal Agent of Collar ROT of Peanut

Twelve isolates of Trichoderma (six of T. harzianum, five of T. viride, one of T. virens), which reduced variably the incidence of collar rot disease caused in peanut by Aspergillus niger Van Tieghem, were evaluated for their potential to produce lytic enzymes during in vitro antagonism. T. viride 6...

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Autores principales: Gajera, H. P., Vakharia, D. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768997/
https://www.ncbi.nlm.nih.gov/pubmed/24031802
http://dx.doi.org/10.1590/S1517-83822012000100005
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author Gajera, H. P.
Vakharia, D. N.
author_facet Gajera, H. P.
Vakharia, D. N.
author_sort Gajera, H. P.
collection PubMed
description Twelve isolates of Trichoderma (six of T. harzianum, five of T. viride, one of T. virens), which reduced variably the incidence of collar rot disease caused in peanut by Aspergillus niger Van Tieghem, were evaluated for their potential to produce lytic enzymes during in vitro antagonism. T. viride 60 inhibited highest (86.2%) growth of test fungus followed by T. harzianum 2J (80.4%) at 6 days after inoculation (DAI) on PDA media. The specific activities of chitinase, β-1,3-glucanase and protease were 11, 3.46 and 9 folds higher in T(6) antagonist (T. viride 60 and A. niger interactions) followed by 8.72, 2.85 and 9 folds in T(8)antagonist (T. harzianum 2J and A. niger interactions), respectively, compared to the activity produced by control petri plate T(13) (A. niger alone) at 6 DAI. Activity of these lytic enzymes induced in antagonists’ plates comprises the growth of Trichoderma isolates. However, cellulase and poly galacturonase were found least amount in these antagonists treatment. A significant positive correlation (p=0.01) between percentage growth inhibition of test fungus and lytic enzymes – (chitinase, β-1,3-glucanase and protease) in the culture medium of antagonist treatment established a relationship to inhibit growth of fungal pathogen by increasing the levels of these enzymes. Among the Trichoderma isolates, T. viride 60 was found best strain to be used in biological control of plant pathogen A. niger.
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spelling pubmed-37689972013-09-12 Production of Lytic Enzymes by Trichoderma Isolates during in vitro Antagonism with Aspergillus Niger, The Causal Agent of Collar ROT of Peanut Gajera, H. P. Vakharia, D. N. Braz J Microbiol Industrial Microbiology Twelve isolates of Trichoderma (six of T. harzianum, five of T. viride, one of T. virens), which reduced variably the incidence of collar rot disease caused in peanut by Aspergillus niger Van Tieghem, were evaluated for their potential to produce lytic enzymes during in vitro antagonism. T. viride 60 inhibited highest (86.2%) growth of test fungus followed by T. harzianum 2J (80.4%) at 6 days after inoculation (DAI) on PDA media. The specific activities of chitinase, β-1,3-glucanase and protease were 11, 3.46 and 9 folds higher in T(6) antagonist (T. viride 60 and A. niger interactions) followed by 8.72, 2.85 and 9 folds in T(8)antagonist (T. harzianum 2J and A. niger interactions), respectively, compared to the activity produced by control petri plate T(13) (A. niger alone) at 6 DAI. Activity of these lytic enzymes induced in antagonists’ plates comprises the growth of Trichoderma isolates. However, cellulase and poly galacturonase were found least amount in these antagonists treatment. A significant positive correlation (p=0.01) between percentage growth inhibition of test fungus and lytic enzymes – (chitinase, β-1,3-glucanase and protease) in the culture medium of antagonist treatment established a relationship to inhibit growth of fungal pathogen by increasing the levels of these enzymes. Among the Trichoderma isolates, T. viride 60 was found best strain to be used in biological control of plant pathogen A. niger. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768997/ /pubmed/24031802 http://dx.doi.org/10.1590/S1517-83822012000100005 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Industrial Microbiology
Gajera, H. P.
Vakharia, D. N.
Production of Lytic Enzymes by Trichoderma Isolates during in vitro Antagonism with Aspergillus Niger, The Causal Agent of Collar ROT of Peanut
title Production of Lytic Enzymes by Trichoderma Isolates during in vitro Antagonism with Aspergillus Niger, The Causal Agent of Collar ROT of Peanut
title_full Production of Lytic Enzymes by Trichoderma Isolates during in vitro Antagonism with Aspergillus Niger, The Causal Agent of Collar ROT of Peanut
title_fullStr Production of Lytic Enzymes by Trichoderma Isolates during in vitro Antagonism with Aspergillus Niger, The Causal Agent of Collar ROT of Peanut
title_full_unstemmed Production of Lytic Enzymes by Trichoderma Isolates during in vitro Antagonism with Aspergillus Niger, The Causal Agent of Collar ROT of Peanut
title_short Production of Lytic Enzymes by Trichoderma Isolates during in vitro Antagonism with Aspergillus Niger, The Causal Agent of Collar ROT of Peanut
title_sort production of lytic enzymes by trichoderma isolates during in vitro antagonism with aspergillus niger, the causal agent of collar rot of peanut
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768997/
https://www.ncbi.nlm.nih.gov/pubmed/24031802
http://dx.doi.org/10.1590/S1517-83822012000100005
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