Cargando…

Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther)

273 bacterial strains were isolated from 20 Chinese longsnout catfish samples. The biochemical characteristics of all strains conformed to the species description of Aeromonas veronii bv. veronii on the basis of Vitek GNI+ card. Furthermore, 16S rDNA, gyrB and rpoD sequences of the representative st...

Descripción completa

Detalles Bibliográficos
Autores principales: Cai, Shuang-Hu, Wu, Zao-He, Jian, Ji-Chang, Lu, Yi-Shan, Tang, Ju-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768999/
https://www.ncbi.nlm.nih.gov/pubmed/24031843
http://dx.doi.org/10.1590/S1517-838220120001000046
_version_ 1782283917883080704
author Cai, Shuang-Hu
Wu, Zao-He
Jian, Ji-Chang
Lu, Yi-Shan
Tang, Ju-Feng
author_facet Cai, Shuang-Hu
Wu, Zao-He
Jian, Ji-Chang
Lu, Yi-Shan
Tang, Ju-Feng
author_sort Cai, Shuang-Hu
collection PubMed
description 273 bacterial strains were isolated from 20 Chinese longsnout catfish samples. The biochemical characteristics of all strains conformed to the species description of Aeromonas veronii bv. veronii on the basis of Vitek GNI+ card. Furthermore, 16S rDNA, gyrB and rpoD sequences of the representative strain PY50 were sequenced and showed high similarity with A. veronii bv. veronii in Genbank. Antibiotic-resistance of the representative strain PY50 was assessed by the Kirby-Bauer disk diffusion method, and the results showed it was susceptible and moderately susceptible to 13 and 4 of the 21 antimicrobial agents tested. Extracellular products of strain PY50 contained gelatinase, lecithinase, elastase, most of lipase and lipopolysaccharide. Virulence of strain PY50 and extracellular products to Chinese longsnout catfish were also tested, and LD(50) were about 3.47×10(4) CFU per fish and 11.22 μg per fish in intraperitoneal injection respectively. This is the first report that A. veronii bv. veronii was the pathogenic agent of ulcerative syndrome in Chinese longsnout catfish.
format Online
Article
Text
id pubmed-3768999
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37689992013-09-12 Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther) Cai, Shuang-Hu Wu, Zao-He Jian, Ji-Chang Lu, Yi-Shan Tang, Ju-Feng Braz J Microbiol Veterinary Microbiology 273 bacterial strains were isolated from 20 Chinese longsnout catfish samples. The biochemical characteristics of all strains conformed to the species description of Aeromonas veronii bv. veronii on the basis of Vitek GNI+ card. Furthermore, 16S rDNA, gyrB and rpoD sequences of the representative strain PY50 were sequenced and showed high similarity with A. veronii bv. veronii in Genbank. Antibiotic-resistance of the representative strain PY50 was assessed by the Kirby-Bauer disk diffusion method, and the results showed it was susceptible and moderately susceptible to 13 and 4 of the 21 antimicrobial agents tested. Extracellular products of strain PY50 contained gelatinase, lecithinase, elastase, most of lipase and lipopolysaccharide. Virulence of strain PY50 and extracellular products to Chinese longsnout catfish were also tested, and LD(50) were about 3.47×10(4) CFU per fish and 11.22 μg per fish in intraperitoneal injection respectively. This is the first report that A. veronii bv. veronii was the pathogenic agent of ulcerative syndrome in Chinese longsnout catfish. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3768999/ /pubmed/24031843 http://dx.doi.org/10.1590/S1517-838220120001000046 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
Cai, Shuang-Hu
Wu, Zao-He
Jian, Ji-Chang
Lu, Yi-Shan
Tang, Ju-Feng
Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther)
title Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther)
title_full Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther)
title_fullStr Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther)
title_full_unstemmed Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther)
title_short Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (Leiocassis longirostris Günther)
title_sort characterization of pathogenic aeromonas veronii bv. veronii associated with ulcerative syndrome from chinese longsnout catfish (leiocassis longirostris günther)
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768999/
https://www.ncbi.nlm.nih.gov/pubmed/24031843
http://dx.doi.org/10.1590/S1517-838220120001000046
work_keys_str_mv AT caishuanghu characterizationofpathogenicaeromonasveroniibvveroniiassociatedwithulcerativesyndromefromchineselongsnoutcatfishleiocassislongirostrisgunther
AT wuzaohe characterizationofpathogenicaeromonasveroniibvveroniiassociatedwithulcerativesyndromefromchineselongsnoutcatfishleiocassislongirostrisgunther
AT jianjichang characterizationofpathogenicaeromonasveroniibvveroniiassociatedwithulcerativesyndromefromchineselongsnoutcatfishleiocassislongirostrisgunther
AT luyishan characterizationofpathogenicaeromonasveroniibvveroniiassociatedwithulcerativesyndromefromchineselongsnoutcatfishleiocassislongirostrisgunther
AT tangjufeng characterizationofpathogenicaeromonasveroniibvveroniiassociatedwithulcerativesyndromefromchineselongsnoutcatfishleiocassislongirostrisgunther