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Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus

We have established methodology for the isolation and characterization of a novel endophytic fungus from the inner bark of medicinal plant Nothapodytes foetida, which produced camptothecin in Sabouraud broth (SB) under shake flask conditions. Camptothecin and its related compounds are at present obt...

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Autores principales: Amna, Touseef, Amina, Musarat, Sharma, P.R., Puri, S.C., Al-Youssef, Hanan M., Al-Taweel, Areej M., Qazi, G. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769012/
https://www.ncbi.nlm.nih.gov/pubmed/24031979
http://dx.doi.org/10.1590/S1517-838220120004000032
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author Amna, Touseef
Amina, Musarat
Sharma, P.R.
Puri, S.C.
Al-Youssef, Hanan M.
Al-Taweel, Areej M.
Qazi, G. N.
author_facet Amna, Touseef
Amina, Musarat
Sharma, P.R.
Puri, S.C.
Al-Youssef, Hanan M.
Al-Taweel, Areej M.
Qazi, G. N.
author_sort Amna, Touseef
collection PubMed
description We have established methodology for the isolation and characterization of a novel endophytic fungus from the inner bark of medicinal plant Nothapodytes foetida, which produced camptothecin in Sabouraud broth (SB) under shake flask conditions. Camptothecin and its related compounds are at present obtained by extraction from intact plants, but fungal endopytes may be an alternative source of production. In present study we have observed the effect of different nutrient combinations and precursors (tryptophan, tryptamine, geraniol, citral, mevalonic acid and leucine) on the accumulation of camptothecin by endophytic fungus Entrophospora infrequens. The precursors were fed either alone or in combinations (tryptophan and geraniol, tryptophan and citral, tryptophan and mevalonic acid, tryptophan and leucine). The highest camptothecin content was observed in the range of 503 ± 25µg/100g dry cell mass in Sabouraud medium. Camptothecin content in the medium was increased by 2.5 folds by the presence of tryptophan and leucine whereas the production with trytophan was also significantly different from other treatments. Furthermore, the effect of fungal camptothecin on the morphology of human cancer cell lines was also studied. The treated cells showed reduction in size, condensation of nucleus and the protoplasmic extensions were reduced. All these characteristics are found in apoptotic cells.
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spelling pubmed-37690122013-09-12 Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus Amna, Touseef Amina, Musarat Sharma, P.R. Puri, S.C. Al-Youssef, Hanan M. Al-Taweel, Areej M. Qazi, G. N. Braz J Microbiol Industrial Microbiology We have established methodology for the isolation and characterization of a novel endophytic fungus from the inner bark of medicinal plant Nothapodytes foetida, which produced camptothecin in Sabouraud broth (SB) under shake flask conditions. Camptothecin and its related compounds are at present obtained by extraction from intact plants, but fungal endopytes may be an alternative source of production. In present study we have observed the effect of different nutrient combinations and precursors (tryptophan, tryptamine, geraniol, citral, mevalonic acid and leucine) on the accumulation of camptothecin by endophytic fungus Entrophospora infrequens. The precursors were fed either alone or in combinations (tryptophan and geraniol, tryptophan and citral, tryptophan and mevalonic acid, tryptophan and leucine). The highest camptothecin content was observed in the range of 503 ± 25µg/100g dry cell mass in Sabouraud medium. Camptothecin content in the medium was increased by 2.5 folds by the presence of tryptophan and leucine whereas the production with trytophan was also significantly different from other treatments. Furthermore, the effect of fungal camptothecin on the morphology of human cancer cell lines was also studied. The treated cells showed reduction in size, condensation of nucleus and the protoplasmic extensions were reduced. All these characteristics are found in apoptotic cells. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3769012/ /pubmed/24031979 http://dx.doi.org/10.1590/S1517-838220120004000032 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Industrial Microbiology
Amna, Touseef
Amina, Musarat
Sharma, P.R.
Puri, S.C.
Al-Youssef, Hanan M.
Al-Taweel, Areej M.
Qazi, G. N.
Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus
title Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus
title_full Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus
title_fullStr Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus
title_full_unstemmed Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus
title_short Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus
title_sort effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus
topic Industrial Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769012/
https://www.ncbi.nlm.nih.gov/pubmed/24031979
http://dx.doi.org/10.1590/S1517-838220120004000032
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