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Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus
We have established methodology for the isolation and characterization of a novel endophytic fungus from the inner bark of medicinal plant Nothapodytes foetida, which produced camptothecin in Sabouraud broth (SB) under shake flask conditions. Camptothecin and its related compounds are at present obt...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769012/ https://www.ncbi.nlm.nih.gov/pubmed/24031979 http://dx.doi.org/10.1590/S1517-838220120004000032 |
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author | Amna, Touseef Amina, Musarat Sharma, P.R. Puri, S.C. Al-Youssef, Hanan M. Al-Taweel, Areej M. Qazi, G. N. |
author_facet | Amna, Touseef Amina, Musarat Sharma, P.R. Puri, S.C. Al-Youssef, Hanan M. Al-Taweel, Areej M. Qazi, G. N. |
author_sort | Amna, Touseef |
collection | PubMed |
description | We have established methodology for the isolation and characterization of a novel endophytic fungus from the inner bark of medicinal plant Nothapodytes foetida, which produced camptothecin in Sabouraud broth (SB) under shake flask conditions. Camptothecin and its related compounds are at present obtained by extraction from intact plants, but fungal endopytes may be an alternative source of production. In present study we have observed the effect of different nutrient combinations and precursors (tryptophan, tryptamine, geraniol, citral, mevalonic acid and leucine) on the accumulation of camptothecin by endophytic fungus Entrophospora infrequens. The precursors were fed either alone or in combinations (tryptophan and geraniol, tryptophan and citral, tryptophan and mevalonic acid, tryptophan and leucine). The highest camptothecin content was observed in the range of 503 ± 25µg/100g dry cell mass in Sabouraud medium. Camptothecin content in the medium was increased by 2.5 folds by the presence of tryptophan and leucine whereas the production with trytophan was also significantly different from other treatments. Furthermore, the effect of fungal camptothecin on the morphology of human cancer cell lines was also studied. The treated cells showed reduction in size, condensation of nucleus and the protoplasmic extensions were reduced. All these characteristics are found in apoptotic cells. |
format | Online Article Text |
id | pubmed-3769012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37690122013-09-12 Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus Amna, Touseef Amina, Musarat Sharma, P.R. Puri, S.C. Al-Youssef, Hanan M. Al-Taweel, Areej M. Qazi, G. N. Braz J Microbiol Industrial Microbiology We have established methodology for the isolation and characterization of a novel endophytic fungus from the inner bark of medicinal plant Nothapodytes foetida, which produced camptothecin in Sabouraud broth (SB) under shake flask conditions. Camptothecin and its related compounds are at present obtained by extraction from intact plants, but fungal endopytes may be an alternative source of production. In present study we have observed the effect of different nutrient combinations and precursors (tryptophan, tryptamine, geraniol, citral, mevalonic acid and leucine) on the accumulation of camptothecin by endophytic fungus Entrophospora infrequens. The precursors were fed either alone or in combinations (tryptophan and geraniol, tryptophan and citral, tryptophan and mevalonic acid, tryptophan and leucine). The highest camptothecin content was observed in the range of 503 ± 25µg/100g dry cell mass in Sabouraud medium. Camptothecin content in the medium was increased by 2.5 folds by the presence of tryptophan and leucine whereas the production with trytophan was also significantly different from other treatments. Furthermore, the effect of fungal camptothecin on the morphology of human cancer cell lines was also studied. The treated cells showed reduction in size, condensation of nucleus and the protoplasmic extensions were reduced. All these characteristics are found in apoptotic cells. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3769012/ /pubmed/24031979 http://dx.doi.org/10.1590/S1517-838220120004000032 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Industrial Microbiology Amna, Touseef Amina, Musarat Sharma, P.R. Puri, S.C. Al-Youssef, Hanan M. Al-Taweel, Areej M. Qazi, G. N. Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus |
title | Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus |
title_full | Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus |
title_fullStr | Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus |
title_full_unstemmed | Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus |
title_short | Effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus |
title_sort | effect of precursors feeding and media manipulation on production of novel anticancer pro-drug camptothecin from endophytic fungus |
topic | Industrial Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769012/ https://www.ncbi.nlm.nih.gov/pubmed/24031979 http://dx.doi.org/10.1590/S1517-838220120004000032 |
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