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Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.]
This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability t...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769046/ https://www.ncbi.nlm.nih.gov/pubmed/24031992 http://dx.doi.org/10.1590/S1517-838220120004000045 |
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author | Júnior, Paulo Ivan Fernandes de Lima, Andréa Aparecida Passos, Samuel Ribeiro Tuão Gava, Carlos Alberto de Oliveira, Paulo Jansen Rumjanek, Norma Gouvêa Xavier, Gustavo Ribeiro |
author_facet | Júnior, Paulo Ivan Fernandes de Lima, Andréa Aparecida Passos, Samuel Ribeiro Tuão Gava, Carlos Alberto de Oliveira, Paulo Jansen Rumjanek, Norma Gouvêa Xavier, Gustavo Ribeiro |
author_sort | Júnior, Paulo Ivan Fernandes |
collection | PubMed |
description | This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization. |
format | Online Article Text |
id | pubmed-3769046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37690462013-09-12 Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.] Júnior, Paulo Ivan Fernandes de Lima, Andréa Aparecida Passos, Samuel Ribeiro Tuão Gava, Carlos Alberto de Oliveira, Paulo Jansen Rumjanek, Norma Gouvêa Xavier, Gustavo Ribeiro Braz J Microbiol Microbial Physiology This study evaluated 26 pigeonpea rhizobial isolates according to their cultural characteristics, intrinsic antibiotic resistance, salt and temperature tolerance, carbon source utilization and amylolytic activity. The cultural characterization showed that the majority of them presented the ability to acidify the YMA. Among the 27 isolates evaluated, 25 were able to grow when incubated at 42° C and 11 showed tolerance to 3% (w/v) of NaCl in YMA medium. The patterns of carbon sources utilization was very diverse among the isolates. It was observed the capacity of three strains to metabolize all the carbon sources evaluated and a total of 42% of the bacterial isolates was able to grow in the culture medium supplemented with at least, six carbon sources. The carbon sources mannitol (control) and sucrose were metabilized by all isolates evaluated. The profile of intrinsic resistance to antibiotics showed that the isolates were mostly resistant to streptomycin and ampicillin, but susceptible to kanamycin and chloranphenicol. High amylolytic activity of, at least, four isolates was also demonstrated, especially for isolated 47.3b, which showed the highest enzymatic index. These results indicate the metabolic versatility of the pigeonpea rhizobia, and indicates the isolate 47.3b to further studies regarding the amylase production and characterization. Sociedade Brasileira de Microbiologia 2012 2012-06-01 /pmc/articles/PMC3769046/ /pubmed/24031992 http://dx.doi.org/10.1590/S1517-838220120004000045 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Microbial Physiology Júnior, Paulo Ivan Fernandes de Lima, Andréa Aparecida Passos, Samuel Ribeiro Tuão Gava, Carlos Alberto de Oliveira, Paulo Jansen Rumjanek, Norma Gouvêa Xavier, Gustavo Ribeiro Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.] |
title | Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.] |
title_full | Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.] |
title_fullStr | Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.] |
title_full_unstemmed | Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.] |
title_short | Phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [Cajanus cajan (L.) Millsp.] |
title_sort | phenotypic diversity and amylolytic activity of fast growing rhizobia from pigeonpea [cajanus cajan (l.) millsp.] |
topic | Microbial Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769046/ https://www.ncbi.nlm.nih.gov/pubmed/24031992 http://dx.doi.org/10.1590/S1517-838220120004000045 |
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