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Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification

The distinction between lymphatic and blood vessels is biologically fundamental. Here we wanted to rigorously analyze the universal applicability of vascular markers and characteristics of the two widely used vascular model systems human microvascular endothelial cell line-1 (HMEC-1) and telomerase-...

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Autores principales: Keuschnigg, Johannes, Karinen, Sirkku, Auvinen, Kaisa, Irjala, Heikki, Mpindi, John-Patrick, Kallioniemi, Olli, Hautaniemi, Sampsa, Jalkanen, Sirpa, Salmi, Marko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769239/
https://www.ncbi.nlm.nih.gov/pubmed/24058540
http://dx.doi.org/10.1371/journal.pone.0074293
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author Keuschnigg, Johannes
Karinen, Sirkku
Auvinen, Kaisa
Irjala, Heikki
Mpindi, John-Patrick
Kallioniemi, Olli
Hautaniemi, Sampsa
Jalkanen, Sirpa
Salmi, Marko
author_facet Keuschnigg, Johannes
Karinen, Sirkku
Auvinen, Kaisa
Irjala, Heikki
Mpindi, John-Patrick
Kallioniemi, Olli
Hautaniemi, Sampsa
Jalkanen, Sirpa
Salmi, Marko
author_sort Keuschnigg, Johannes
collection PubMed
description The distinction between lymphatic and blood vessels is biologically fundamental. Here we wanted to rigorously analyze the universal applicability of vascular markers and characteristics of the two widely used vascular model systems human microvascular endothelial cell line-1 (HMEC-1) and telomerase-immortalized microvascular endothelial cell line (TIME). Therefore we studied the protein expression and functional properties of the endothelial cell lines HMEC-1 and TIME by flow cytometry and in vitro flow assays. We then performed microarray analyses of the gene expression in these two cell lines and compared them to primary endothelial cells. Using bioinformatics we then defined 39 new, more universal, endothelial-type specific markers from 47 primary endothelial microarray datasets and validated them using immunohistochemistry with normal and pathological tissues. We surprisingly found that both HMEC-1 and TIME are hybrid blood- and lymphatic cells. In addition, we discovered great discrepancies in the previous identifications of blood- and lymphatic endothelium-specific genes. Hence we identified and validated new, universally applicable vascular markers. Summarizing, the hybrid blood-lymphatic endothelial phenotype of HMEC-1 and TIME is indicative of plasticity in the gene expression of immortalized endothelial cell lines. Moreover, we identified new, stable, vessel-type specific markers for blood- and lymphatic endothelium, useful for basic research and clinical diagnostics.
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spelling pubmed-37692392013-09-20 Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification Keuschnigg, Johannes Karinen, Sirkku Auvinen, Kaisa Irjala, Heikki Mpindi, John-Patrick Kallioniemi, Olli Hautaniemi, Sampsa Jalkanen, Sirpa Salmi, Marko PLoS One Research Article The distinction between lymphatic and blood vessels is biologically fundamental. Here we wanted to rigorously analyze the universal applicability of vascular markers and characteristics of the two widely used vascular model systems human microvascular endothelial cell line-1 (HMEC-1) and telomerase-immortalized microvascular endothelial cell line (TIME). Therefore we studied the protein expression and functional properties of the endothelial cell lines HMEC-1 and TIME by flow cytometry and in vitro flow assays. We then performed microarray analyses of the gene expression in these two cell lines and compared them to primary endothelial cells. Using bioinformatics we then defined 39 new, more universal, endothelial-type specific markers from 47 primary endothelial microarray datasets and validated them using immunohistochemistry with normal and pathological tissues. We surprisingly found that both HMEC-1 and TIME are hybrid blood- and lymphatic cells. In addition, we discovered great discrepancies in the previous identifications of blood- and lymphatic endothelium-specific genes. Hence we identified and validated new, universally applicable vascular markers. Summarizing, the hybrid blood-lymphatic endothelial phenotype of HMEC-1 and TIME is indicative of plasticity in the gene expression of immortalized endothelial cell lines. Moreover, we identified new, stable, vessel-type specific markers for blood- and lymphatic endothelium, useful for basic research and clinical diagnostics. Public Library of Science 2013-09-10 /pmc/articles/PMC3769239/ /pubmed/24058540 http://dx.doi.org/10.1371/journal.pone.0074293 Text en © 2013 Keuschnigg et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Keuschnigg, Johannes
Karinen, Sirkku
Auvinen, Kaisa
Irjala, Heikki
Mpindi, John-Patrick
Kallioniemi, Olli
Hautaniemi, Sampsa
Jalkanen, Sirpa
Salmi, Marko
Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification
title Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification
title_full Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification
title_fullStr Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification
title_full_unstemmed Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification
title_short Plasticity of Blood- and Lymphatic Endothelial Cells and Marker Identification
title_sort plasticity of blood- and lymphatic endothelial cells and marker identification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769239/
https://www.ncbi.nlm.nih.gov/pubmed/24058540
http://dx.doi.org/10.1371/journal.pone.0074293
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