Cargando…
A Small Conductance Calcium-Activated K(+) Channel in C. elegans, KCNL-2, Plays a Role in the Regulation of the Rate of Egg-Laying
In the nervous system of mice, small conductance calcium-activated potassium (SK) channels function to regulate neuronal excitability through the generation of a component of the medium afterhyperpolarization that follows action potentials. In humans, irregular action potential firing frequency unde...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769271/ https://www.ncbi.nlm.nih.gov/pubmed/24040423 http://dx.doi.org/10.1371/journal.pone.0075869 |
_version_ | 1782283956387840000 |
---|---|
author | Chotoo, Cavita K. Silverman, Gary A. Devor, Daniel C. Luke, Cliff J. |
author_facet | Chotoo, Cavita K. Silverman, Gary A. Devor, Daniel C. Luke, Cliff J. |
author_sort | Chotoo, Cavita K. |
collection | PubMed |
description | In the nervous system of mice, small conductance calcium-activated potassium (SK) channels function to regulate neuronal excitability through the generation of a component of the medium afterhyperpolarization that follows action potentials. In humans, irregular action potential firing frequency underlies diseases such as ataxia, epilepsy, schizophrenia and Parkinson’s disease. Due to the complexity of studying protein function in the mammalian nervous system, we sought to characterize an SK channel homologue, KCNL-2, in C. elegans, a genetically tractable system in which the lineage of individual neurons was mapped from their early developmental stages. Sequence analysis of the KCNL-2 protein reveals that the six transmembrane domains, the potassium-selective pore and the calmodulin binding domain are highly conserved with the mammalian homologues. We used widefield and confocal fluorescent imaging to show that a fusion construct of KCNL-2 with GFP in transgenic lines is expressed in the nervous system of C. elegans. We also show that a KCNL-2 null strain, kcnl-2(tm1885), demonstrates a mild egg-laying defective phenotype, a phenotype that is rescued in a KCNL-2-dependent manner. Conversely, we show that transgenic lines that overexpress KCNL-2 demonstrate a hyperactive egg-laying phenotype. In this study, we show that the vulva of transgenic hermaphrodites is highly innervated by neuronal processes and by the VC4 and VC5 neurons that express GFP-tagged KCNL-2. We propose that KCNL-2 functions in the nervous system of C. elegans to regulate the rate of egg-laying. |
format | Online Article Text |
id | pubmed-3769271 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37692712013-09-13 A Small Conductance Calcium-Activated K(+) Channel in C. elegans, KCNL-2, Plays a Role in the Regulation of the Rate of Egg-Laying Chotoo, Cavita K. Silverman, Gary A. Devor, Daniel C. Luke, Cliff J. PLoS One Research Article In the nervous system of mice, small conductance calcium-activated potassium (SK) channels function to regulate neuronal excitability through the generation of a component of the medium afterhyperpolarization that follows action potentials. In humans, irregular action potential firing frequency underlies diseases such as ataxia, epilepsy, schizophrenia and Parkinson’s disease. Due to the complexity of studying protein function in the mammalian nervous system, we sought to characterize an SK channel homologue, KCNL-2, in C. elegans, a genetically tractable system in which the lineage of individual neurons was mapped from their early developmental stages. Sequence analysis of the KCNL-2 protein reveals that the six transmembrane domains, the potassium-selective pore and the calmodulin binding domain are highly conserved with the mammalian homologues. We used widefield and confocal fluorescent imaging to show that a fusion construct of KCNL-2 with GFP in transgenic lines is expressed in the nervous system of C. elegans. We also show that a KCNL-2 null strain, kcnl-2(tm1885), demonstrates a mild egg-laying defective phenotype, a phenotype that is rescued in a KCNL-2-dependent manner. Conversely, we show that transgenic lines that overexpress KCNL-2 demonstrate a hyperactive egg-laying phenotype. In this study, we show that the vulva of transgenic hermaphrodites is highly innervated by neuronal processes and by the VC4 and VC5 neurons that express GFP-tagged KCNL-2. We propose that KCNL-2 functions in the nervous system of C. elegans to regulate the rate of egg-laying. Public Library of Science 2013-09-10 /pmc/articles/PMC3769271/ /pubmed/24040423 http://dx.doi.org/10.1371/journal.pone.0075869 Text en © 2013 Chotoo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chotoo, Cavita K. Silverman, Gary A. Devor, Daniel C. Luke, Cliff J. A Small Conductance Calcium-Activated K(+) Channel in C. elegans, KCNL-2, Plays a Role in the Regulation of the Rate of Egg-Laying |
title | A Small Conductance Calcium-Activated K(+) Channel in C. elegans, KCNL-2, Plays a Role in the Regulation of the Rate of Egg-Laying |
title_full | A Small Conductance Calcium-Activated K(+) Channel in C. elegans, KCNL-2, Plays a Role in the Regulation of the Rate of Egg-Laying |
title_fullStr | A Small Conductance Calcium-Activated K(+) Channel in C. elegans, KCNL-2, Plays a Role in the Regulation of the Rate of Egg-Laying |
title_full_unstemmed | A Small Conductance Calcium-Activated K(+) Channel in C. elegans, KCNL-2, Plays a Role in the Regulation of the Rate of Egg-Laying |
title_short | A Small Conductance Calcium-Activated K(+) Channel in C. elegans, KCNL-2, Plays a Role in the Regulation of the Rate of Egg-Laying |
title_sort | small conductance calcium-activated k(+) channel in c. elegans, kcnl-2, plays a role in the regulation of the rate of egg-laying |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769271/ https://www.ncbi.nlm.nih.gov/pubmed/24040423 http://dx.doi.org/10.1371/journal.pone.0075869 |
work_keys_str_mv | AT chotoocavitak asmallconductancecalciumactivatedkchannelinceleganskcnl2playsaroleintheregulationoftherateofegglaying AT silvermangarya asmallconductancecalciumactivatedkchannelinceleganskcnl2playsaroleintheregulationoftherateofegglaying AT devordanielc asmallconductancecalciumactivatedkchannelinceleganskcnl2playsaroleintheregulationoftherateofegglaying AT lukecliffj asmallconductancecalciumactivatedkchannelinceleganskcnl2playsaroleintheregulationoftherateofegglaying AT chotoocavitak smallconductancecalciumactivatedkchannelinceleganskcnl2playsaroleintheregulationoftherateofegglaying AT silvermangarya smallconductancecalciumactivatedkchannelinceleganskcnl2playsaroleintheregulationoftherateofegglaying AT devordanielc smallconductancecalciumactivatedkchannelinceleganskcnl2playsaroleintheregulationoftherateofegglaying AT lukecliffj smallconductancecalciumactivatedkchannelinceleganskcnl2playsaroleintheregulationoftherateofegglaying |