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Cell- Cell Transmission of VSV-G Pseudotyped Lentivector Particles

Many replicating viruses, including HIV-1 and HTLV-1, are efficiently transmitted from the cell surface of actively infected cells upon contact with bystander cells. In a previous study, we reported the prolonged cell surface retention of VSV-G replication-deficient pseudotyped lentivector prior to...

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Detalles Bibliográficos
Autores principales: Skinner, Amy M., Chakkaramakkil Verghese, Santhosh, Kurre, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769293/
https://www.ncbi.nlm.nih.gov/pubmed/24040363
http://dx.doi.org/10.1371/journal.pone.0074925
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author Skinner, Amy M.
Chakkaramakkil Verghese, Santhosh
Kurre, Peter
author_facet Skinner, Amy M.
Chakkaramakkil Verghese, Santhosh
Kurre, Peter
author_sort Skinner, Amy M.
collection PubMed
description Many replicating viruses, including HIV-1 and HTLV-1, are efficiently transmitted from the cell surface of actively infected cells upon contact with bystander cells. In a previous study, we reported the prolonged cell surface retention of VSV-G replication-deficient pseudotyped lentivector prior to endocytic entry. However, the competing kinetics of cell surface versus dissociation, neutralization or direct transfer to other cells have received comparatively little attention. Here we demonstrate that the relative efficiency of cell-cell surface transmission can outpace “cell-free” transduction at limiting vector input. This coincides with the prolonged half-life of cell bound vector but occurs, unlike HTLV-1, without evidence for particle aggregation. These studies suggest that cell-surface attachment stabilizes particles and alters neutralization kinetics. Our experiments provide novel insight into the underexplored cell-cell transmission of pseudotyped particles.
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spelling pubmed-37692932013-09-13 Cell- Cell Transmission of VSV-G Pseudotyped Lentivector Particles Skinner, Amy M. Chakkaramakkil Verghese, Santhosh Kurre, Peter PLoS One Research Article Many replicating viruses, including HIV-1 and HTLV-1, are efficiently transmitted from the cell surface of actively infected cells upon contact with bystander cells. In a previous study, we reported the prolonged cell surface retention of VSV-G replication-deficient pseudotyped lentivector prior to endocytic entry. However, the competing kinetics of cell surface versus dissociation, neutralization or direct transfer to other cells have received comparatively little attention. Here we demonstrate that the relative efficiency of cell-cell surface transmission can outpace “cell-free” transduction at limiting vector input. This coincides with the prolonged half-life of cell bound vector but occurs, unlike HTLV-1, without evidence for particle aggregation. These studies suggest that cell-surface attachment stabilizes particles and alters neutralization kinetics. Our experiments provide novel insight into the underexplored cell-cell transmission of pseudotyped particles. Public Library of Science 2013-09-10 /pmc/articles/PMC3769293/ /pubmed/24040363 http://dx.doi.org/10.1371/journal.pone.0074925 Text en © 2013 Skinner et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Skinner, Amy M.
Chakkaramakkil Verghese, Santhosh
Kurre, Peter
Cell- Cell Transmission of VSV-G Pseudotyped Lentivector Particles
title Cell- Cell Transmission of VSV-G Pseudotyped Lentivector Particles
title_full Cell- Cell Transmission of VSV-G Pseudotyped Lentivector Particles
title_fullStr Cell- Cell Transmission of VSV-G Pseudotyped Lentivector Particles
title_full_unstemmed Cell- Cell Transmission of VSV-G Pseudotyped Lentivector Particles
title_short Cell- Cell Transmission of VSV-G Pseudotyped Lentivector Particles
title_sort cell- cell transmission of vsv-g pseudotyped lentivector particles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769293/
https://www.ncbi.nlm.nih.gov/pubmed/24040363
http://dx.doi.org/10.1371/journal.pone.0074925
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