Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes

Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different loca...

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Detalles Bibliográficos
Autores principales: Cho, Jung-Hee, Lee, Seung-Eun, Chang, Who-Bong, Cha, Jae-Soon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2006
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769545/
https://www.ncbi.nlm.nih.gov/pubmed/24039479
http://dx.doi.org/10.4489/MYCO.2006.34.2.104
Descripción
Sumario:Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom.

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