Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes

Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different loca...

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Autores principales: Cho, Jung-Hee, Lee, Seung-Eun, Chang, Who-Bong, Cha, Jae-Soon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Mycology 2006
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769545/
https://www.ncbi.nlm.nih.gov/pubmed/24039479
http://dx.doi.org/10.4489/MYCO.2006.34.2.104
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author Cho, Jung-Hee
Lee, Seung-Eun
Chang, Who-Bong
Cha, Jae-Soon
author_facet Cho, Jung-Hee
Lee, Seung-Eun
Chang, Who-Bong
Cha, Jae-Soon
author_sort Cho, Jung-Hee
collection PubMed
description Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom.
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spelling pubmed-37695452013-09-13 Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes Cho, Jung-Hee Lee, Seung-Eun Chang, Who-Bong Cha, Jae-Soon Mycobiology Note Flammulina velutipes was transformed efficiently by Agrobacterium-mediated transformation system. The transformation frequency was about 16% with the gill tissues of the fungal fruiting body. Southern hybridization and genetic analysis suggest that the introduced DNA was inserted onto different locations of the fungal genome, and inherited stably to the next generation via basidiospores. Transformation or gene tagging with Agrobacterium T-DNA based vector should be useful for wide ranges of genetic or molecular biological studies of the mushroom. The Korean Society of Mycology 2006-06 2006-06-30 /pmc/articles/PMC3769545/ /pubmed/24039479 http://dx.doi.org/10.4489/MYCO.2006.34.2.104 Text en Copyright © 2006 by The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Note
Cho, Jung-Hee
Lee, Seung-Eun
Chang, Who-Bong
Cha, Jae-Soon
Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes
title Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes
title_full Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes
title_fullStr Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes
title_full_unstemmed Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes
title_short Agrobacterium-mediated Transformation of the Winter Mushroom, Flammulina velutipes
title_sort agrobacterium-mediated transformation of the winter mushroom, flammulina velutipes
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769545/
https://www.ncbi.nlm.nih.gov/pubmed/24039479
http://dx.doi.org/10.4489/MYCO.2006.34.2.104
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