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The Fruiting Body Formation of Armillaria mellea on Oak Sawdust Medium Covered with Ground Raw Carrots
To produce an artificial fruiting body of Armillaria mellea on the oak sawdust medium, seven strains of A. mellea were used. The top surface of oak sawdust medium covered with ground raw carrot was inoculated with each of 7 strains and cultured for 30 days at 25℃ in the dark condition until the myce...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Mycology
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769575/ https://www.ncbi.nlm.nih.gov/pubmed/24039500 http://dx.doi.org/10.4489/MYCO.2006.34.4.206 |
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author | Shim, Jae-Ouk Chang, Kwang-Choon Lee, Youn-Su Park, Cheol-Ho Kim, Hey-Young Lee, U-Youn Lee, Tae-Soo Lee, Min-Woong |
author_facet | Shim, Jae-Ouk Chang, Kwang-Choon Lee, Youn-Su Park, Cheol-Ho Kim, Hey-Young Lee, U-Youn Lee, Tae-Soo Lee, Min-Woong |
author_sort | Shim, Jae-Ouk |
collection | PubMed |
description | To produce an artificial fruiting body of Armillaria mellea on the oak sawdust medium, seven strains of A. mellea were used. The top surface of oak sawdust medium covered with ground raw carrot was inoculated with each of 7 strains and cultured for 30 days at 25℃ in the dark condition until the mycelia of A. mellea completely colonized the medium from top to bottom. Then, the mycelia which were fully covered on the top surface of the medium were scratched slightly with a spatula and filled with tap water for 3 hours. To induce the primordial formation, the 7 strains of A. mellea were transferred to the growth chamber under the illumination (350 lux) of 12 hours and relative humidity of 85 ± 5% in a day and then cultured at 16 ± 1℃. Only A. mellea IUM 949 could form primordia on the sawdust medium, but the other strains did not make primordia at the same condition. The primordia of A. mellea IUM 949 were formed 10 days after complete colonization of the medium and the fruiting bodies were produced 7 days after a primordial formation. The experimental results suggested that IUM 949 strain might be a good candidate for mass production of fruiting bodies of A. mellea. |
format | Online Article Text |
id | pubmed-3769575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Korean Society of Mycology |
record_format | MEDLINE/PubMed |
spelling | pubmed-37695752013-09-13 The Fruiting Body Formation of Armillaria mellea on Oak Sawdust Medium Covered with Ground Raw Carrots Shim, Jae-Ouk Chang, Kwang-Choon Lee, Youn-Su Park, Cheol-Ho Kim, Hey-Young Lee, U-Youn Lee, Tae-Soo Lee, Min-Woong Mycobiology Research Article To produce an artificial fruiting body of Armillaria mellea on the oak sawdust medium, seven strains of A. mellea were used. The top surface of oak sawdust medium covered with ground raw carrot was inoculated with each of 7 strains and cultured for 30 days at 25℃ in the dark condition until the mycelia of A. mellea completely colonized the medium from top to bottom. Then, the mycelia which were fully covered on the top surface of the medium were scratched slightly with a spatula and filled with tap water for 3 hours. To induce the primordial formation, the 7 strains of A. mellea were transferred to the growth chamber under the illumination (350 lux) of 12 hours and relative humidity of 85 ± 5% in a day and then cultured at 16 ± 1℃. Only A. mellea IUM 949 could form primordia on the sawdust medium, but the other strains did not make primordia at the same condition. The primordia of A. mellea IUM 949 were formed 10 days after complete colonization of the medium and the fruiting bodies were produced 7 days after a primordial formation. The experimental results suggested that IUM 949 strain might be a good candidate for mass production of fruiting bodies of A. mellea. The Korean Society of Mycology 2006-12 2006-12-31 /pmc/articles/PMC3769575/ /pubmed/24039500 http://dx.doi.org/10.4489/MYCO.2006.34.4.206 Text en Copyright © 2006 by The Korean Society of Mycology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Shim, Jae-Ouk Chang, Kwang-Choon Lee, Youn-Su Park, Cheol-Ho Kim, Hey-Young Lee, U-Youn Lee, Tae-Soo Lee, Min-Woong The Fruiting Body Formation of Armillaria mellea on Oak Sawdust Medium Covered with Ground Raw Carrots |
title | The Fruiting Body Formation of Armillaria mellea on Oak Sawdust Medium Covered with Ground Raw Carrots |
title_full | The Fruiting Body Formation of Armillaria mellea on Oak Sawdust Medium Covered with Ground Raw Carrots |
title_fullStr | The Fruiting Body Formation of Armillaria mellea on Oak Sawdust Medium Covered with Ground Raw Carrots |
title_full_unstemmed | The Fruiting Body Formation of Armillaria mellea on Oak Sawdust Medium Covered with Ground Raw Carrots |
title_short | The Fruiting Body Formation of Armillaria mellea on Oak Sawdust Medium Covered with Ground Raw Carrots |
title_sort | fruiting body formation of armillaria mellea on oak sawdust medium covered with ground raw carrots |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769575/ https://www.ncbi.nlm.nih.gov/pubmed/24039500 http://dx.doi.org/10.4489/MYCO.2006.34.4.206 |
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