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The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells

PURPOSE: The purpose of the current study was to examine the effect of dexamethasone (Dex) at various concentrations on the apoptosis and mineralization of human periodontal ligament (hPDL) cells. METHODS: hPDL cells were obtained from the mid-third of premolars extracted for orthodontic reasons, an...

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Autores principales: Kim, Sung-Mi, Kim, Yong-Gun, Park, Jin-Woo, Lee, Jae-Mok, Suh, Jo-Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Academy of Periodontology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769595/
https://www.ncbi.nlm.nih.gov/pubmed/24040569
http://dx.doi.org/10.5051/jpis.2013.43.4.168
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author Kim, Sung-Mi
Kim, Yong-Gun
Park, Jin-Woo
Lee, Jae-Mok
Suh, Jo-Young
author_facet Kim, Sung-Mi
Kim, Yong-Gun
Park, Jin-Woo
Lee, Jae-Mok
Suh, Jo-Young
author_sort Kim, Sung-Mi
collection PubMed
description PURPOSE: The purpose of the current study was to examine the effect of dexamethasone (Dex) at various concentrations on the apoptosis and mineralization of human periodontal ligament (hPDL) cells. METHODS: hPDL cells were obtained from the mid-third of premolars extracted for orthodontic reasons, and a primary culture of hPDL cells was prepared using an explant technique. Groups of cells were divided according to the concentration of Dex (0, 1, 10, 100, and 1,000 nM). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed for evaluation of cellular viability, and alkaline phosphatase activity was examined for osteogenic differentiation of hPDL cells. Alizarin Red S staining was performed for observation of mineralization, and real-time polymerase chain reaction was performed for the evaluation of related genes. RESULTS: Increasing the Dex concentration was found to reduce cellular viability, with an increase in alkaline phosphatase activity and mineralization. Within the range of Dex concentrations tested in this study, 100 nM of Dex was found to promote the most vigorous differentiation and mineralization of hPDL cells. Dex-induced osteogenic differentiation and mineralization was accompanied by an increase in the level of osteogenic and apoptosis-related genes and a reduction in the level of antiapoptotic genes. The decrease in hPDL cellular viability by glucocorticoid may be explained in part by the increased prevalence of cell apoptosis, as demonstrated by BAX expression and decreased expression of the antiapoptotic gene, Bcl-2. CONCLUSIONS: An increase in hPDL cell differentiation rather than cellular viability at an early stage is likely to be a key factor in glucocorticoid induced mineralization. In addition, apoptosis might play an important role in Dex-induced tissue regeneration; however, further study is needed for investigation of the precise mechanism.
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spelling pubmed-37695952013-09-13 The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells Kim, Sung-Mi Kim, Yong-Gun Park, Jin-Woo Lee, Jae-Mok Suh, Jo-Young J Periodontal Implant Sci Research Article PURPOSE: The purpose of the current study was to examine the effect of dexamethasone (Dex) at various concentrations on the apoptosis and mineralization of human periodontal ligament (hPDL) cells. METHODS: hPDL cells were obtained from the mid-third of premolars extracted for orthodontic reasons, and a primary culture of hPDL cells was prepared using an explant technique. Groups of cells were divided according to the concentration of Dex (0, 1, 10, 100, and 1,000 nM). A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed for evaluation of cellular viability, and alkaline phosphatase activity was examined for osteogenic differentiation of hPDL cells. Alizarin Red S staining was performed for observation of mineralization, and real-time polymerase chain reaction was performed for the evaluation of related genes. RESULTS: Increasing the Dex concentration was found to reduce cellular viability, with an increase in alkaline phosphatase activity and mineralization. Within the range of Dex concentrations tested in this study, 100 nM of Dex was found to promote the most vigorous differentiation and mineralization of hPDL cells. Dex-induced osteogenic differentiation and mineralization was accompanied by an increase in the level of osteogenic and apoptosis-related genes and a reduction in the level of antiapoptotic genes. The decrease in hPDL cellular viability by glucocorticoid may be explained in part by the increased prevalence of cell apoptosis, as demonstrated by BAX expression and decreased expression of the antiapoptotic gene, Bcl-2. CONCLUSIONS: An increase in hPDL cell differentiation rather than cellular viability at an early stage is likely to be a key factor in glucocorticoid induced mineralization. In addition, apoptosis might play an important role in Dex-induced tissue regeneration; however, further study is needed for investigation of the precise mechanism. Korean Academy of Periodontology 2013-08 2013-08-31 /pmc/articles/PMC3769595/ /pubmed/24040569 http://dx.doi.org/10.5051/jpis.2013.43.4.168 Text en Copyright © 2013 Korean Academy of Periodontology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).
spellingShingle Research Article
Kim, Sung-Mi
Kim, Yong-Gun
Park, Jin-Woo
Lee, Jae-Mok
Suh, Jo-Young
The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells
title The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells
title_full The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells
title_fullStr The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells
title_full_unstemmed The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells
title_short The effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells
title_sort effects of dexamethasone on the apoptosis and osteogenic differentiation of human periodontal ligament cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769595/
https://www.ncbi.nlm.nih.gov/pubmed/24040569
http://dx.doi.org/10.5051/jpis.2013.43.4.168
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