Cargando…

Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses

Members of the Enterobacteriaceae family are present in the intestines of man and animals as commensals or are important disease causing agents. Bacteria bearing multidrug efflux systems (MDR) are able to survive adverse ecological niches. Multiresistant Escherichia coli and Enterobacter cloacae iso...

Descripción completa

Detalles Bibliográficos
Autores principales: Moreira, Maria Aparecida S., Rodrigues, Patrícia P.C.F., Tomaz, Rafael S., de Moraes, Célia A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769709/
https://www.ncbi.nlm.nih.gov/pubmed/24031352
http://dx.doi.org/10.1590/S1517-83822009000200007
_version_ 1782284017214685184
author Moreira, Maria Aparecida S.
Rodrigues, Patrícia P.C.F.
Tomaz, Rafael S.
de Moraes, Célia A.
author_facet Moreira, Maria Aparecida S.
Rodrigues, Patrícia P.C.F.
Tomaz, Rafael S.
de Moraes, Célia A.
author_sort Moreira, Maria Aparecida S.
collection PubMed
description Members of the Enterobacteriaceae family are present in the intestines of man and animals as commensals or are important disease causing agents. Bacteria bearing multidrug efflux systems (MDR) are able to survive adverse ecological niches. Multiresistant Escherichia coli and Enterobacter cloacae isolates from wholesome broiler carcasses were investigated for the presence of MDR. Lowering of Minimal Inhibitory Concentration for antimicrobials in the presence of a proton-motive force (PMF) uncoupler was tested as a potential display of the MDR phenotype. PCR amplification of the genes encoding AcrA and AcrB, components of a MDR system was performed. Diversity of each species was ascertained by Pulsed-Field Gel Electrophoresis (PFGE) of DNA digested with endonuclease XbaI. For all the isolates, except E. coli 1 and E. cloacae 9, lowering of MIC or of the growth rate in the presence of antimicrobials was observed, indicating a PMF dependent resistance mechanism. Expected products of DNA amplification with acrAB derived primers was obtained with all E. coli strains and with two of the five E. cloacae strains. Dendrogram generated shows diverse pulsetypes, confirming the genetic diversity among the strains. An important issue and related public health is the fact that different models and mechanisms of antimicrobial resistance are present in a small number of non-pathogenic strains and isolated from the same origin. These may be sources of resistance genes to others microorganisms, among them, pathogenic strains.
format Online
Article
Text
id pubmed-3769709
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37697092013-09-12 Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses Moreira, Maria Aparecida S. Rodrigues, Patrícia P.C.F. Tomaz, Rafael S. de Moraes, Célia A. Braz J Microbiol Veterinary Microbiology Members of the Enterobacteriaceae family are present in the intestines of man and animals as commensals or are important disease causing agents. Bacteria bearing multidrug efflux systems (MDR) are able to survive adverse ecological niches. Multiresistant Escherichia coli and Enterobacter cloacae isolates from wholesome broiler carcasses were investigated for the presence of MDR. Lowering of Minimal Inhibitory Concentration for antimicrobials in the presence of a proton-motive force (PMF) uncoupler was tested as a potential display of the MDR phenotype. PCR amplification of the genes encoding AcrA and AcrB, components of a MDR system was performed. Diversity of each species was ascertained by Pulsed-Field Gel Electrophoresis (PFGE) of DNA digested with endonuclease XbaI. For all the isolates, except E. coli 1 and E. cloacae 9, lowering of MIC or of the growth rate in the presence of antimicrobials was observed, indicating a PMF dependent resistance mechanism. Expected products of DNA amplification with acrAB derived primers was obtained with all E. coli strains and with two of the five E. cloacae strains. Dendrogram generated shows diverse pulsetypes, confirming the genetic diversity among the strains. An important issue and related public health is the fact that different models and mechanisms of antimicrobial resistance are present in a small number of non-pathogenic strains and isolated from the same origin. These may be sources of resistance genes to others microorganisms, among them, pathogenic strains. Sociedade Brasileira de Microbiologia 2009 2009-06-01 /pmc/articles/PMC3769709/ /pubmed/24031352 http://dx.doi.org/10.1590/S1517-83822009000200007 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Veterinary Microbiology
Moreira, Maria Aparecida S.
Rodrigues, Patrícia P.C.F.
Tomaz, Rafael S.
de Moraes, Célia A.
Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses
title Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses
title_full Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses
title_fullStr Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses
title_full_unstemmed Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses
title_short Multidrug efflux systems in Escherichia coli and Enterobacter cloacae obtained from wholesome broiler carcasses
title_sort multidrug efflux systems in escherichia coli and enterobacter cloacae obtained from wholesome broiler carcasses
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769709/
https://www.ncbi.nlm.nih.gov/pubmed/24031352
http://dx.doi.org/10.1590/S1517-83822009000200007
work_keys_str_mv AT moreiramariaaparecidas multidrugeffluxsystemsinescherichiacoliandenterobactercloacaeobtainedfromwholesomebroilercarcasses
AT rodriguespatriciapcf multidrugeffluxsystemsinescherichiacoliandenterobactercloacaeobtainedfromwholesomebroilercarcasses
AT tomazrafaels multidrugeffluxsystemsinescherichiacoliandenterobactercloacaeobtainedfromwholesomebroilercarcasses
AT demoraesceliaa multidrugeffluxsystemsinescherichiacoliandenterobactercloacaeobtainedfromwholesomebroilercarcasses