In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells

A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from in...

Descripción completa

Detalles Bibliográficos
Autores principales: Bastos, Camila V., Passos, Lygia M. F., Vasconcelos, Maria Mercês C., Ribeiro, Múcio F. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2009
Materias:
General Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769711/
https://www.ncbi.nlm.nih.gov/pubmed/24031379
http://dx.doi.org/10.1590/S1517-838220090002000034
_version_ 1782284017686544384
author Bastos, Camila V.
Passos, Lygia M. F.
Vasconcelos, Maria Mercês C.
Ribeiro, Múcio F. B.
author_facet Bastos, Camila V.
Passos, Lygia M. F.
Vasconcelos, Maria Mercês C.
Ribeiro, Múcio F. B.
author_sort Bastos, Camila V.
collection PubMed
description A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from infected IDE8 cells. Comparisons with partial sequences of the msp1α gene and the complete genome of A. marginale confirmed that the sequences of amplified fragments were from the A. marginale genome. This is the first establishment of a Brazilian A. marginale isolate in tick cells, representing a new system for biological and molecular studies and also a new source of material for diagnosis and development of vaccines.
format Online
Article
Text
id pubmed-3769711
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37697112013-09-12 In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells Bastos, Camila V. Passos, Lygia M. F. Vasconcelos, Maria Mercês C. Ribeiro, Múcio F. B. Braz J Microbiol General Microbiology A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from infected IDE8 cells. Comparisons with partial sequences of the msp1α gene and the complete genome of A. marginale confirmed that the sequences of amplified fragments were from the A. marginale genome. This is the first establishment of a Brazilian A. marginale isolate in tick cells, representing a new system for biological and molecular studies and also a new source of material for diagnosis and development of vaccines. Sociedade Brasileira de Microbiologia 2009 2009-06-01 /pmc/articles/PMC3769711/ /pubmed/24031379 http://dx.doi.org/10.1590/S1517-838220090002000034 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle General Microbiology
Bastos, Camila V.
Passos, Lygia M. F.
Vasconcelos, Maria Mercês C.
Ribeiro, Múcio F. B.
In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells
title In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells
title_full In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells
title_fullStr In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells
title_full_unstemmed In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells
title_short In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells
title_sort in vitro establishment and propagation of a brazilian strain of anaplasma marginale with appendage in ide8 (ixodes scapularis) cells
topic General Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769711/
https://www.ncbi.nlm.nih.gov/pubmed/24031379
http://dx.doi.org/10.1590/S1517-838220090002000034
work_keys_str_mv AT bastoscamilav invitroestablishmentandpropagationofabrazilianstrainofanaplasmamarginalewithappendageinide8ixodesscapulariscells
AT passoslygiamf invitroestablishmentandpropagationofabrazilianstrainofanaplasmamarginalewithappendageinide8ixodesscapulariscells
AT vasconcelosmariamercesc invitroestablishmentandpropagationofabrazilianstrainofanaplasmamarginalewithappendageinide8ixodesscapulariscells
AT ribeiromuciofb invitroestablishmentandpropagationofabrazilianstrainofanaplasmamarginalewithappendageinide8ixodesscapulariscells

Ejemplares similares