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In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells
A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from in...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769711/ https://www.ncbi.nlm.nih.gov/pubmed/24031379 http://dx.doi.org/10.1590/S1517-838220090002000034 |
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author | Bastos, Camila V. Passos, Lygia M. F. Vasconcelos, Maria Mercês C. Ribeiro, Múcio F. B. |
author_facet | Bastos, Camila V. Passos, Lygia M. F. Vasconcelos, Maria Mercês C. Ribeiro, Múcio F. B. |
author_sort | Bastos, Camila V. |
collection | PubMed |
description | A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from infected IDE8 cells. Comparisons with partial sequences of the msp1α gene and the complete genome of A. marginale confirmed that the sequences of amplified fragments were from the A. marginale genome. This is the first establishment of a Brazilian A. marginale isolate in tick cells, representing a new system for biological and molecular studies and also a new source of material for diagnosis and development of vaccines. |
format | Online Article Text |
id | pubmed-3769711 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37697112013-09-12 In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells Bastos, Camila V. Passos, Lygia M. F. Vasconcelos, Maria Mercês C. Ribeiro, Múcio F. B. Braz J Microbiol General Microbiology A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from infected IDE8 cells. Comparisons with partial sequences of the msp1α gene and the complete genome of A. marginale confirmed that the sequences of amplified fragments were from the A. marginale genome. This is the first establishment of a Brazilian A. marginale isolate in tick cells, representing a new system for biological and molecular studies and also a new source of material for diagnosis and development of vaccines. Sociedade Brasileira de Microbiologia 2009 2009-06-01 /pmc/articles/PMC3769711/ /pubmed/24031379 http://dx.doi.org/10.1590/S1517-838220090002000034 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | General Microbiology Bastos, Camila V. Passos, Lygia M. F. Vasconcelos, Maria Mercês C. Ribeiro, Múcio F. B. In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells |
title | In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells |
title_full | In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells |
title_fullStr | In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells |
title_full_unstemmed | In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells |
title_short | In vitro establishment and propagation of a Brazilian strain of Anaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells |
title_sort | in vitro establishment and propagation of a brazilian strain of anaplasma marginale with appendage in ide8 (ixodes scapularis) cells |
topic | General Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769711/ https://www.ncbi.nlm.nih.gov/pubmed/24031379 http://dx.doi.org/10.1590/S1517-838220090002000034 |
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