Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India

Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis...

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Autores principales: Parvathi, Ammini, Krishna, Kiran, Jose, Jiya, Joseph, Neetha, Nair, Santha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769717/
https://www.ncbi.nlm.nih.gov/pubmed/24031357
http://dx.doi.org/10.1590/S1517-838220090002000012
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author Parvathi, Ammini
Krishna, Kiran
Jose, Jiya
Joseph, Neetha
Nair, Santha
author_facet Parvathi, Ammini
Krishna, Kiran
Jose, Jiya
Joseph, Neetha
Nair, Santha
author_sort Parvathi, Ammini
collection PubMed
description Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase activity and uniformly sensitive to antibiotics tested in this study. One strain of B. pumilus harboured cereulide synthetase gene cesB of B. cereus which was indistinguishable from rest of the isolates biochemically and by AP-PCR. This study reports, for the first time, the presence of the emetic toxin gene cesB in B. pumilus.
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spelling pubmed-37697172013-09-12 Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India Parvathi, Ammini Krishna, Kiran Jose, Jiya Joseph, Neetha Nair, Santha Braz J Microbiol Environmental Microbiology Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase activity and uniformly sensitive to antibiotics tested in this study. One strain of B. pumilus harboured cereulide synthetase gene cesB of B. cereus which was indistinguishable from rest of the isolates biochemically and by AP-PCR. This study reports, for the first time, the presence of the emetic toxin gene cesB in B. pumilus. Sociedade Brasileira de Microbiologia 2009 2009-06-01 /pmc/articles/PMC3769717/ /pubmed/24031357 http://dx.doi.org/10.1590/S1517-838220090002000012 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Environmental Microbiology
Parvathi, Ammini
Krishna, Kiran
Jose, Jiya
Joseph, Neetha
Nair, Santha
Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India
title Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India
title_full Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India
title_fullStr Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India
title_full_unstemmed Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India
title_short Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India
title_sort biochemical and molecular characterization of bacillus pumilus isolated from coastal environment in cochin, india
topic Environmental Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769717/
https://www.ncbi.nlm.nih.gov/pubmed/24031357
http://dx.doi.org/10.1590/S1517-838220090002000012
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