Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India
Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sociedade Brasileira de Microbiologia
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769717/ https://www.ncbi.nlm.nih.gov/pubmed/24031357 http://dx.doi.org/10.1590/S1517-838220090002000012 |
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author | Parvathi, Ammini Krishna, Kiran Jose, Jiya Joseph, Neetha Nair, Santha |
author_facet | Parvathi, Ammini Krishna, Kiran Jose, Jiya Joseph, Neetha Nair, Santha |
author_sort | Parvathi, Ammini |
collection | PubMed |
description | Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase activity and uniformly sensitive to antibiotics tested in this study. One strain of B. pumilus harboured cereulide synthetase gene cesB of B. cereus which was indistinguishable from rest of the isolates biochemically and by AP-PCR. This study reports, for the first time, the presence of the emetic toxin gene cesB in B. pumilus. |
format | Online Article Text |
id | pubmed-3769717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37697172013-09-12 Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India Parvathi, Ammini Krishna, Kiran Jose, Jiya Joseph, Neetha Nair, Santha Braz J Microbiol Environmental Microbiology Bacillus species constitute a diverse group of bacteria widely distributed in soil and the aquatic environment. In this study, Bacillus strains isolated from the coastal environment of Cochin, India were identified by detailed conventional biochemical methods, fatty acid methyl ester (FAME) analysis and partial 16S rDNA sequencing. Analysis of the data revealed that Bacillus pumilus was the most predominant species in the region under study followed by B. cereus and B. sphaericus. The B. pumilus isolates were further characterized by arbitrarily primed PCR (AP-PCR), antibiotic sensitivity profiling and PCR screening for known toxin genes associated with Bacillus spp. All B. pumilus isolates were biochemically identical, exhibited high protease and lipase activity and uniformly sensitive to antibiotics tested in this study. One strain of B. pumilus harboured cereulide synthetase gene cesB of B. cereus which was indistinguishable from rest of the isolates biochemically and by AP-PCR. This study reports, for the first time, the presence of the emetic toxin gene cesB in B. pumilus. Sociedade Brasileira de Microbiologia 2009 2009-06-01 /pmc/articles/PMC3769717/ /pubmed/24031357 http://dx.doi.org/10.1590/S1517-838220090002000012 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Environmental Microbiology Parvathi, Ammini Krishna, Kiran Jose, Jiya Joseph, Neetha Nair, Santha Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India |
title | Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India |
title_full | Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India |
title_fullStr | Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India |
title_full_unstemmed | Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India |
title_short | Biochemical and molecular characterization of Bacillus pumilus isolated from coastal environment in Cochin, India |
title_sort | biochemical and molecular characterization of bacillus pumilus isolated from coastal environment in cochin, india |
topic | Environmental Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769717/ https://www.ncbi.nlm.nih.gov/pubmed/24031357 http://dx.doi.org/10.1590/S1517-838220090002000012 |
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