Identification of Mycobacterium bovis Isolates by a multiplex PCR

Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the iso...

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Detalles Bibliográficos
Autores principales: de Souza Figueiredo, Eduardo Eustáquio, Silvestre, Flávia Galindo, Campos, Wilma Neres, Furlanetto, Leone Vinícius, Medeiros, Luciana, Lilenbaum, Walter, Fonseca, Leila Sousa, Silva, Joab Trajano, Paschoalin, Vânia Margaret Flosi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2009
Materias:
Veterinary Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769727/
https://www.ncbi.nlm.nih.gov/pubmed/24031349
http://dx.doi.org/10.1590/S1517-83822009000200004
Descripción
Sumario:Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates.

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