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Analysis of methane biodegradation by Methylosinus trichosporium OB3b
The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus tr...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Microbiologia
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769742/ https://www.ncbi.nlm.nih.gov/pubmed/24031362 http://dx.doi.org/10.1590/S1517-838220090002000017 |
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author | Rodrigues, Andréa dos Santos Salgado, Belkis Valdman e Andréa Medeiros |
author_facet | Rodrigues, Andréa dos Santos Salgado, Belkis Valdman e Andréa Medeiros |
author_sort | Rodrigues, Andréa dos Santos |
collection | PubMed |
description | The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monitoring methods for methane. The results showed that M. trichosporium OB3b was capable to degrade methane in a more effective way with an initial microorganism concentration of 0.0700 g.L(-1), temperature of 30ºC, pH 6.5 and using 1.79 mmol of methane. In these same conditions, there was no bacterial growth when 2.69 mmol of methane was used. The specific rate of microorganism growth, the conversion factor, the efficiency and the volumetric productivity, for the optimized conditions of biodegradation were, respectively, 0.0324 h(-1), 0.6830 gcells/gCH(4), 73.73% and 2.7732.10(-3) gcells/L.h. The final product of methane microbiological degradation, carbon dioxide, was quantified through the use of a commercial electrode, and, through this, the grade of methane conversion in carbon dioxide was calculated. |
format | Online Article Text |
id | pubmed-3769742 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Sociedade Brasileira de Microbiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-37697422013-09-12 Analysis of methane biodegradation by Methylosinus trichosporium OB3b Rodrigues, Andréa dos Santos Salgado, Belkis Valdman e Andréa Medeiros Braz J Microbiol Environmental Microbiology The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monitoring methods for methane. The results showed that M. trichosporium OB3b was capable to degrade methane in a more effective way with an initial microorganism concentration of 0.0700 g.L(-1), temperature of 30ºC, pH 6.5 and using 1.79 mmol of methane. In these same conditions, there was no bacterial growth when 2.69 mmol of methane was used. The specific rate of microorganism growth, the conversion factor, the efficiency and the volumetric productivity, for the optimized conditions of biodegradation were, respectively, 0.0324 h(-1), 0.6830 gcells/gCH(4), 73.73% and 2.7732.10(-3) gcells/L.h. The final product of methane microbiological degradation, carbon dioxide, was quantified through the use of a commercial electrode, and, through this, the grade of methane conversion in carbon dioxide was calculated. Sociedade Brasileira de Microbiologia 2009 2009-06-01 /pmc/articles/PMC3769742/ /pubmed/24031362 http://dx.doi.org/10.1590/S1517-838220090002000017 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License |
spellingShingle | Environmental Microbiology Rodrigues, Andréa dos Santos Salgado, Belkis Valdman e Andréa Medeiros Analysis of methane biodegradation by Methylosinus trichosporium OB3b |
title | Analysis of methane biodegradation by Methylosinus trichosporium OB3b |
title_full | Analysis of methane biodegradation by Methylosinus trichosporium OB3b |
title_fullStr | Analysis of methane biodegradation by Methylosinus trichosporium OB3b |
title_full_unstemmed | Analysis of methane biodegradation by Methylosinus trichosporium OB3b |
title_short | Analysis of methane biodegradation by Methylosinus trichosporium OB3b |
title_sort | analysis of methane biodegradation by methylosinus trichosporium ob3b |
topic | Environmental Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769742/ https://www.ncbi.nlm.nih.gov/pubmed/24031362 http://dx.doi.org/10.1590/S1517-838220090002000017 |
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