R-Loop Formation In Trans at an AGGAG Repeat

Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was f...

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Detalles Bibliográficos
Autores principales: Toriumi, Kazuya, Tsukahara, Takuma, Hanai, Ryo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770058/
https://www.ncbi.nlm.nih.gov/pubmed/24066229
http://dx.doi.org/10.1155/2013/629218
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author Toriumi, Kazuya
Tsukahara, Takuma
Hanai, Ryo
author_facet Toriumi, Kazuya
Tsukahara, Takuma
Hanai, Ryo
author_sort Toriumi, Kazuya
collection PubMed
description Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling.
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spelling pubmed-37700582013-09-24 R-Loop Formation In Trans at an AGGAG Repeat Toriumi, Kazuya Tsukahara, Takuma Hanai, Ryo J Nucleic Acids Research Article Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling. Hindawi Publishing Corporation 2013 2013-08-26 /pmc/articles/PMC3770058/ /pubmed/24066229 http://dx.doi.org/10.1155/2013/629218 Text en Copyright © 2013 Kazuya Toriumi et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Toriumi, Kazuya
Tsukahara, Takuma
Hanai, Ryo
R-Loop Formation In Trans at an AGGAG Repeat
title R-Loop Formation In Trans at an AGGAG Repeat
title_full R-Loop Formation In Trans at an AGGAG Repeat
title_fullStr R-Loop Formation In Trans at an AGGAG Repeat
title_full_unstemmed R-Loop Formation In Trans at an AGGAG Repeat
title_short R-Loop Formation In Trans at an AGGAG Repeat
title_sort r-loop formation in trans at an aggag repeat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770058/
https://www.ncbi.nlm.nih.gov/pubmed/24066229
http://dx.doi.org/10.1155/2013/629218
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