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Agrobacterium-mediated genetic transformation using cotyledons in Japanese pear (Pyrus pyrifolia)
Genetic transformation was successfully established producing both transformed adventitious shoots and calli in Japanese pear (Pyrus pyrifolia Nakai) by using cotyledons as explants. Cotyledons of five cultivars were co-cultivated with Agrobacterium tumefaciens strain LBA4404 carrying the pBIN19-sgf...
Autores principales: | Nakajima, Ikuko, Sato, Yoshihiko, Saito, Toshihiro, Moriguchi, Takaya, Yamamoto, Toshiya |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Breeding
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770554/ https://www.ncbi.nlm.nih.gov/pubmed/24273422 http://dx.doi.org/10.1270/jsbbs.63.275 |
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