Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F(8) recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DA...

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Detalles Bibliográficos
Autores principales: Vipin, Cina Ann, Luckett, David J., Harper, John D.I., Ash, Gavin J., Kilian, Andrzej, Ellwood, Simon R., Phan, Huyen T.T., Raman, Harsh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Breeding 2013
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770556/
https://www.ncbi.nlm.nih.gov/pubmed/24273424
http://dx.doi.org/10.1270/jsbbs.63.292
Descripción
Sumario:We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an F(8) recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genic markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good genome coverage and were associated with previously identified genic and AFLP markers linked with quantitative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.

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