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Comprehensive Analysis of Genic Male Sterility-Related Genes in Brassica rapa Using a Newly Developed Br300K Oligomeric Chip
To identify genes associated with genic male sterility (GMS) that could be useful for hybrid breeding in Chinese cabbage ( Brassica rapa ssp. pekinensis ), floral bud transcriptome analysis was carried out using a B . rapa microarray with 300,000 probes (Br300K). Among 47,548 clones deposited on a B...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770635/ https://www.ncbi.nlm.nih.gov/pubmed/24039743 http://dx.doi.org/10.1371/journal.pone.0072178 |
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author | Dong, Xiangshu Feng, Hui Xu, Ming Lee, Jeongyeo Kim, Yeon Ki Lim, Yong Pyo Piao, Zhongyun Park, Young Doo Ma, Hong Hur, Yoonkang |
author_facet | Dong, Xiangshu Feng, Hui Xu, Ming Lee, Jeongyeo Kim, Yeon Ki Lim, Yong Pyo Piao, Zhongyun Park, Young Doo Ma, Hong Hur, Yoonkang |
author_sort | Dong, Xiangshu |
collection | PubMed |
description | To identify genes associated with genic male sterility (GMS) that could be useful for hybrid breeding in Chinese cabbage ( Brassica rapa ssp. pekinensis ), floral bud transcriptome analysis was carried out using a B . rapa microarray with 300,000 probes (Br300K). Among 47,548 clones deposited on a Br300K microarray with seven probes of 60 nt length within the 3' 150 bp region, a total of 10,622 genes were differentially expressed between fertile and sterile floral buds; 4,774 and 5,848 genes were up-regulated over 2-fold in fertile and sterile buds, respectively. However, the expression of 1,413 and 199 genes showed fertile and sterile bud-specific features, respectively. Genes expressed specifically in fertile buds, possibly GMS-related genes, included homologs of several Arabidopsis male sterility-related genes, genes associated with the cell wall and synthesis of its surface proteins, pollen wall and coat components, signaling components, and nutrient supplies. However, most early genes for pollen development, genes for primexine and callose formation, and genes for pollen maturation and anther dehiscence showed no difference in expression between fertile and sterile buds. Some of the known genes associated with Arabidopsis pollen development showed similar expression patterns to those seen in this study, while others did not. BrbHLH89 and BrMYP99 are putative GMS genes. Additionally, 17 novel genes identified only in B . rapa were specifically and highly expressed only in fertile buds, implying the possible involvement in male fertility. All data suggest that Chinese cabbage GMS might be controlled by genes acting in post-meiotic tapetal development that are different from those known to be associated with Arabidopsis male sterility. |
format | Online Article Text |
id | pubmed-3770635 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-37706352013-09-13 Comprehensive Analysis of Genic Male Sterility-Related Genes in Brassica rapa Using a Newly Developed Br300K Oligomeric Chip Dong, Xiangshu Feng, Hui Xu, Ming Lee, Jeongyeo Kim, Yeon Ki Lim, Yong Pyo Piao, Zhongyun Park, Young Doo Ma, Hong Hur, Yoonkang PLoS One Research Article To identify genes associated with genic male sterility (GMS) that could be useful for hybrid breeding in Chinese cabbage ( Brassica rapa ssp. pekinensis ), floral bud transcriptome analysis was carried out using a B . rapa microarray with 300,000 probes (Br300K). Among 47,548 clones deposited on a Br300K microarray with seven probes of 60 nt length within the 3' 150 bp region, a total of 10,622 genes were differentially expressed between fertile and sterile floral buds; 4,774 and 5,848 genes were up-regulated over 2-fold in fertile and sterile buds, respectively. However, the expression of 1,413 and 199 genes showed fertile and sterile bud-specific features, respectively. Genes expressed specifically in fertile buds, possibly GMS-related genes, included homologs of several Arabidopsis male sterility-related genes, genes associated with the cell wall and synthesis of its surface proteins, pollen wall and coat components, signaling components, and nutrient supplies. However, most early genes for pollen development, genes for primexine and callose formation, and genes for pollen maturation and anther dehiscence showed no difference in expression between fertile and sterile buds. Some of the known genes associated with Arabidopsis pollen development showed similar expression patterns to those seen in this study, while others did not. BrbHLH89 and BrMYP99 are putative GMS genes. Additionally, 17 novel genes identified only in B . rapa were specifically and highly expressed only in fertile buds, implying the possible involvement in male fertility. All data suggest that Chinese cabbage GMS might be controlled by genes acting in post-meiotic tapetal development that are different from those known to be associated with Arabidopsis male sterility. Public Library of Science 2013-09-11 /pmc/articles/PMC3770635/ /pubmed/24039743 http://dx.doi.org/10.1371/journal.pone.0072178 Text en © 2013 Dong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Dong, Xiangshu Feng, Hui Xu, Ming Lee, Jeongyeo Kim, Yeon Ki Lim, Yong Pyo Piao, Zhongyun Park, Young Doo Ma, Hong Hur, Yoonkang Comprehensive Analysis of Genic Male Sterility-Related Genes in Brassica rapa Using a Newly Developed Br300K Oligomeric Chip |
title | Comprehensive Analysis of Genic Male Sterility-Related Genes in Brassica
rapa Using a Newly Developed Br300K Oligomeric Chip |
title_full | Comprehensive Analysis of Genic Male Sterility-Related Genes in Brassica
rapa Using a Newly Developed Br300K Oligomeric Chip |
title_fullStr | Comprehensive Analysis of Genic Male Sterility-Related Genes in Brassica
rapa Using a Newly Developed Br300K Oligomeric Chip |
title_full_unstemmed | Comprehensive Analysis of Genic Male Sterility-Related Genes in Brassica
rapa Using a Newly Developed Br300K Oligomeric Chip |
title_short | Comprehensive Analysis of Genic Male Sterility-Related Genes in Brassica
rapa Using a Newly Developed Br300K Oligomeric Chip |
title_sort | comprehensive analysis of genic male sterility-related genes in brassica
rapa using a newly developed br300k oligomeric chip |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770635/ https://www.ncbi.nlm.nih.gov/pubmed/24039743 http://dx.doi.org/10.1371/journal.pone.0072178 |
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