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Integrative Analysis of Porcine microRNAome during Skeletal Muscle Development

Pig is an important agricultural animal for meat production and provides a valuable model for many human diseases. Functional studies have demonstrated that microRNAs (miRNAs) play critical roles in almost all aspects of skeletal muscle development and disease pathogenesis. To investigate the miRNAs...

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Autores principales: Qin, Lijun, Chen, Yaosheng, Liu, Xiaohong, Ye, Sanxing, Yu, Kaifan, Huang, Zheng, Yu, Jingwei, Zhou, Xingyu, Chen, Hu, Mo, Delin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770649/
https://www.ncbi.nlm.nih.gov/pubmed/24039761
http://dx.doi.org/10.1371/journal.pone.0072418
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author Qin, Lijun
Chen, Yaosheng
Liu, Xiaohong
Ye, Sanxing
Yu, Kaifan
Huang, Zheng
Yu, Jingwei
Zhou, Xingyu
Chen, Hu
Mo, Delin
author_facet Qin, Lijun
Chen, Yaosheng
Liu, Xiaohong
Ye, Sanxing
Yu, Kaifan
Huang, Zheng
Yu, Jingwei
Zhou, Xingyu
Chen, Hu
Mo, Delin
author_sort Qin, Lijun
collection PubMed
description Pig is an important agricultural animal for meat production and provides a valuable model for many human diseases. Functional studies have demonstrated that microRNAs (miRNAs) play critical roles in almost all aspects of skeletal muscle development and disease pathogenesis. To investigate the miRNAs involved in regulating different periods of skeletal muscle development, we herein performed a comprehensive research for porcine microRNAome (miRNAome) during 10 skeletal muscle developmental stages including 35, 49, 63, 77, 91 dpc (days post coitum) and 2, 28, 90, 120, 180 dpn (days postnatal) using Solexa sequencing technology. Our results extend the repertoire of pig miRNAome to 247 known miRNAs processed from 210 pre-miRNAs and 297 candidate novel miRNAs through comparison with known miRNAs in the miRBase. Expression analysis of the 15 most abundant miRNAs in every library indicated that functional miRNAome may be smaller and tend to be highly expressed. A series of muscle-related miRNAs summarized in our study present different patterns between myofibers formation phase and muscle maturation phase, providing valuable reference for investigation of functional miRNAs during skeletal muscle development. Analysis of temporal profiles of miRNA expression identifies 18 novel candidate myogenic miRNAs in pig, which might provide new insight into regulation mechanism mediated by miRNAs underlying muscle development.
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spelling pubmed-37706492013-09-13 Integrative Analysis of Porcine microRNAome during Skeletal Muscle Development Qin, Lijun Chen, Yaosheng Liu, Xiaohong Ye, Sanxing Yu, Kaifan Huang, Zheng Yu, Jingwei Zhou, Xingyu Chen, Hu Mo, Delin PLoS One Research Article Pig is an important agricultural animal for meat production and provides a valuable model for many human diseases. Functional studies have demonstrated that microRNAs (miRNAs) play critical roles in almost all aspects of skeletal muscle development and disease pathogenesis. To investigate the miRNAs involved in regulating different periods of skeletal muscle development, we herein performed a comprehensive research for porcine microRNAome (miRNAome) during 10 skeletal muscle developmental stages including 35, 49, 63, 77, 91 dpc (days post coitum) and 2, 28, 90, 120, 180 dpn (days postnatal) using Solexa sequencing technology. Our results extend the repertoire of pig miRNAome to 247 known miRNAs processed from 210 pre-miRNAs and 297 candidate novel miRNAs through comparison with known miRNAs in the miRBase. Expression analysis of the 15 most abundant miRNAs in every library indicated that functional miRNAome may be smaller and tend to be highly expressed. A series of muscle-related miRNAs summarized in our study present different patterns between myofibers formation phase and muscle maturation phase, providing valuable reference for investigation of functional miRNAs during skeletal muscle development. Analysis of temporal profiles of miRNA expression identifies 18 novel candidate myogenic miRNAs in pig, which might provide new insight into regulation mechanism mediated by miRNAs underlying muscle development. Public Library of Science 2013-09-11 /pmc/articles/PMC3770649/ /pubmed/24039761 http://dx.doi.org/10.1371/journal.pone.0072418 Text en © 2013 Qin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Qin, Lijun
Chen, Yaosheng
Liu, Xiaohong
Ye, Sanxing
Yu, Kaifan
Huang, Zheng
Yu, Jingwei
Zhou, Xingyu
Chen, Hu
Mo, Delin
Integrative Analysis of Porcine microRNAome during Skeletal Muscle Development
title Integrative Analysis of Porcine microRNAome during Skeletal Muscle Development
title_full Integrative Analysis of Porcine microRNAome during Skeletal Muscle Development
title_fullStr Integrative Analysis of Porcine microRNAome during Skeletal Muscle Development
title_full_unstemmed Integrative Analysis of Porcine microRNAome during Skeletal Muscle Development
title_short Integrative Analysis of Porcine microRNAome during Skeletal Muscle Development
title_sort integrative analysis of porcine micrornaome during skeletal muscle development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770649/
https://www.ncbi.nlm.nih.gov/pubmed/24039761
http://dx.doi.org/10.1371/journal.pone.0072418
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