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Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis

Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O(2)(.-)) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated wit...

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Detalles Bibliográficos
Autores principales: Song, Hyun-Ouk, Ryu, Jae-Sook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Parasitology and Tropical Medicine 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770882/
https://www.ncbi.nlm.nih.gov/pubmed/24039294
http://dx.doi.org/10.3347/kjp.2013.51.4.479
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author Song, Hyun-Ouk
Ryu, Jae-Sook
author_facet Song, Hyun-Ouk
Ryu, Jae-Sook
author_sort Song, Hyun-Ouk
collection PubMed
description Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O(2)(.-)) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ESP). To assess the role of trichomonad protease in production of superoxide anions by neutrophils, T. vaginalis lysate, ESP, and MC were each pretreated with a protease inhibitor cocktail before incubation with neutrophils. Superoxide anion production was significantly decreased by this treatment. Trichomonad growth was inhibited by preincubation with supernatants of neutrophils incubated for 3 hr with T. vaginalis lysate. Furthermore, myeloperoxidase (MPO) production by neutrophils was stimulated by live trichomonads. These results indicate that the production of superoxide anions and MPO by neutrophils stimulated with T. vaginalis may be a part of defense mechanisms of neutrophils in trichomoniasis.
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spelling pubmed-37708822013-09-13 Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis Song, Hyun-Ouk Ryu, Jae-Sook Korean J Parasitol Brief Communication Neutrophils are the predominant inflammatory cells found in vaginal discharges of patients infected with Trichomonas vaginalis. In this study, we examined superoxide anion (O(2)(.-)) production by neutrophils activated by T. vaginalis. Human neutrophils produced superoxide anions when stimulated with either a lysate of T. vaginalis, its membrane component (MC), or excretory-secretory product (ESP). To assess the role of trichomonad protease in production of superoxide anions by neutrophils, T. vaginalis lysate, ESP, and MC were each pretreated with a protease inhibitor cocktail before incubation with neutrophils. Superoxide anion production was significantly decreased by this treatment. Trichomonad growth was inhibited by preincubation with supernatants of neutrophils incubated for 3 hr with T. vaginalis lysate. Furthermore, myeloperoxidase (MPO) production by neutrophils was stimulated by live trichomonads. These results indicate that the production of superoxide anions and MPO by neutrophils stimulated with T. vaginalis may be a part of defense mechanisms of neutrophils in trichomoniasis. The Korean Society for Parasitology and Tropical Medicine 2013-08 2013-08-30 /pmc/articles/PMC3770882/ /pubmed/24039294 http://dx.doi.org/10.3347/kjp.2013.51.4.479 Text en © 2013, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Song, Hyun-Ouk
Ryu, Jae-Sook
Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
title Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
title_full Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
title_fullStr Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
title_full_unstemmed Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
title_short Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
title_sort superoxide anion production by human neutrophils activated by trichomonas vaginalis
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3770882/
https://www.ncbi.nlm.nih.gov/pubmed/24039294
http://dx.doi.org/10.3347/kjp.2013.51.4.479
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