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Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo

The aim of myocardial tissue engineering is to repair or regenerate damaged myocardium with engineered cardiac tissue. However, this strategy has been hampered by lack of functional integration of grafts with native myocardium. Autonomic innervation may be crucial for grafts to function properly wit...

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Autores principales: Fu, Xian-ming, Lee, Jong-Kook, Miwa, Keiko, Shimizu, Tatsuya, Takagishi, Yoshiko, Hirabayashi, Masumi, Watabe, Kazuhiko, Usui, Akihiko, Kodama, Itsuo, Ueda, Yuichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771253/
https://www.ncbi.nlm.nih.gov/pubmed/24066291
http://dx.doi.org/10.1155/2013/532720
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author Fu, Xian-ming
Lee, Jong-Kook
Miwa, Keiko
Shimizu, Tatsuya
Takagishi, Yoshiko
Hirabayashi, Masumi
Watabe, Kazuhiko
Usui, Akihiko
Kodama, Itsuo
Ueda, Yuichi
author_facet Fu, Xian-ming
Lee, Jong-Kook
Miwa, Keiko
Shimizu, Tatsuya
Takagishi, Yoshiko
Hirabayashi, Masumi
Watabe, Kazuhiko
Usui, Akihiko
Kodama, Itsuo
Ueda, Yuichi
author_sort Fu, Xian-ming
collection PubMed
description The aim of myocardial tissue engineering is to repair or regenerate damaged myocardium with engineered cardiac tissue. However, this strategy has been hampered by lack of functional integration of grafts with native myocardium. Autonomic innervation may be crucial for grafts to function properly with host myocardium. In this study, we explored the feasibility of in vivo induction of autonomic innervation to engineered myocardial tissue using genetic modulation by adenovirus encoding glial cell line derived neurotrophic factor (GDNF). GFP-transgene (control group) or GDNF overexpressing (GDNF group) engineered cardiomyocyte sheets were transplanted on cryoinjured hearts in rats. Nerve fibers in the grafts were examined by immunohistochemistry at 1, 2, and 4 weeks postoperatively. Growth associated protein-43 positive growing nerves and tyrosine hydroxylase positive sympathetic nerves were first detected in the grafts at 2 weeks postoperatively in control group and 1 week in GDNF group. The densities of growing nerve and sympathetic nerve in grafts were significantly increased in GDNF group. No choline acetyltransferase immunopositive parasympathetic nerves were observed in grafts. In conclusion, sympathetic innervation could be effectively induced into engrafted engineered cardiomyocyte sheets using GDNF.
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spelling pubmed-37712532013-09-24 Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo Fu, Xian-ming Lee, Jong-Kook Miwa, Keiko Shimizu, Tatsuya Takagishi, Yoshiko Hirabayashi, Masumi Watabe, Kazuhiko Usui, Akihiko Kodama, Itsuo Ueda, Yuichi Biomed Res Int Research Article The aim of myocardial tissue engineering is to repair or regenerate damaged myocardium with engineered cardiac tissue. However, this strategy has been hampered by lack of functional integration of grafts with native myocardium. Autonomic innervation may be crucial for grafts to function properly with host myocardium. In this study, we explored the feasibility of in vivo induction of autonomic innervation to engineered myocardial tissue using genetic modulation by adenovirus encoding glial cell line derived neurotrophic factor (GDNF). GFP-transgene (control group) or GDNF overexpressing (GDNF group) engineered cardiomyocyte sheets were transplanted on cryoinjured hearts in rats. Nerve fibers in the grafts were examined by immunohistochemistry at 1, 2, and 4 weeks postoperatively. Growth associated protein-43 positive growing nerves and tyrosine hydroxylase positive sympathetic nerves were first detected in the grafts at 2 weeks postoperatively in control group and 1 week in GDNF group. The densities of growing nerve and sympathetic nerve in grafts were significantly increased in GDNF group. No choline acetyltransferase immunopositive parasympathetic nerves were observed in grafts. In conclusion, sympathetic innervation could be effectively induced into engrafted engineered cardiomyocyte sheets using GDNF. Hindawi Publishing Corporation 2013 2013-08-28 /pmc/articles/PMC3771253/ /pubmed/24066291 http://dx.doi.org/10.1155/2013/532720 Text en Copyright © 2013 Xian-ming Fu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fu, Xian-ming
Lee, Jong-Kook
Miwa, Keiko
Shimizu, Tatsuya
Takagishi, Yoshiko
Hirabayashi, Masumi
Watabe, Kazuhiko
Usui, Akihiko
Kodama, Itsuo
Ueda, Yuichi
Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo
title Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo
title_full Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo
title_fullStr Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo
title_full_unstemmed Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo
title_short Sympathetic Innervation Induced in Engrafted Engineered Cardiomyocyte Sheets by Glial Cell Line Derived Neurotrophic Factor In Vivo
title_sort sympathetic innervation induced in engrafted engineered cardiomyocyte sheets by glial cell line derived neurotrophic factor in vivo
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771253/
https://www.ncbi.nlm.nih.gov/pubmed/24066291
http://dx.doi.org/10.1155/2013/532720
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