Cargando…

Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.

In recent years, there has been a great deal of attention toward free radicals, reactive oxygen species (ROS) generated by exposure of crop plant cells to physical radiations. Henceforth, the current study was planned to compare oxidative stress and mutagenic potential of different irradiation doses...

Descripción completa

Detalles Bibliográficos
Autores principales: Abdel Haliem, Ekram, Abdullah, Hanan, AL-Huqail, Asma A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771406/
https://www.ncbi.nlm.nih.gov/pubmed/24066298
http://dx.doi.org/10.1155/2013/824656
_version_ 1782284191243698176
author Abdel Haliem, Ekram
Abdullah, Hanan
AL-Huqail, Asma A.
author_facet Abdel Haliem, Ekram
Abdullah, Hanan
AL-Huqail, Asma A.
author_sort Abdel Haliem, Ekram
collection PubMed
description In recent years, there has been a great deal of attention toward free radicals, reactive oxygen species (ROS) generated by exposure of crop plant cells to physical radiations. Henceforth, the current study was planned to compare oxidative stress and mutagenic potential of different irradiation doses of fast neutron (FN) and UV-B on meiotic-pollen mother cells (PMCs), pollen grains (PGs) and seeds yielded from irradiated faba beans seedlings. On the cytogenetic level, each irradiation type had special interference with DNA of PMC and exhibited wide range of mutagenic action on the frequency and type of chromosomal anomalies, fertility of PGs and seed yield productivity based on the irradiation exposure dose and radiation sensitivity of faba bean plants compared with un-irradiated ones. On the molecular level, SDS-PAGE and RPAD-PCR analyses of seeds yielded from irradiated seedlings exhibited distinctive polymorphisms based on size, intensity, appearance, and disappearance of polypeptides bands compared with un-irradiated ones. The total values of protein and DNA polymorphisms reached 88% and 90.80% respectively. The neutron fluency (2.3 × 10(6) n/cm(2)) and UV-B dose for 1 hr were recorded as bio-positive effects. The present study proved that genetic variations revealed by cytogenetic test could be supported by gene expression (alterations in RAPD and protein profiles).
format Online
Article
Text
id pubmed-3771406
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-37714062013-09-24 Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L. Abdel Haliem, Ekram Abdullah, Hanan AL-Huqail, Asma A. Biomed Res Int Research Article In recent years, there has been a great deal of attention toward free radicals, reactive oxygen species (ROS) generated by exposure of crop plant cells to physical radiations. Henceforth, the current study was planned to compare oxidative stress and mutagenic potential of different irradiation doses of fast neutron (FN) and UV-B on meiotic-pollen mother cells (PMCs), pollen grains (PGs) and seeds yielded from irradiated faba beans seedlings. On the cytogenetic level, each irradiation type had special interference with DNA of PMC and exhibited wide range of mutagenic action on the frequency and type of chromosomal anomalies, fertility of PGs and seed yield productivity based on the irradiation exposure dose and radiation sensitivity of faba bean plants compared with un-irradiated ones. On the molecular level, SDS-PAGE and RPAD-PCR analyses of seeds yielded from irradiated seedlings exhibited distinctive polymorphisms based on size, intensity, appearance, and disappearance of polypeptides bands compared with un-irradiated ones. The total values of protein and DNA polymorphisms reached 88% and 90.80% respectively. The neutron fluency (2.3 × 10(6) n/cm(2)) and UV-B dose for 1 hr were recorded as bio-positive effects. The present study proved that genetic variations revealed by cytogenetic test could be supported by gene expression (alterations in RAPD and protein profiles). Hindawi Publishing Corporation 2013 2013-08-28 /pmc/articles/PMC3771406/ /pubmed/24066298 http://dx.doi.org/10.1155/2013/824656 Text en Copyright © 2013 Ekram Abdel Haliem et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Abdel Haliem, Ekram
Abdullah, Hanan
AL-Huqail, Asma A.
Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.
title Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.
title_full Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.
title_fullStr Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.
title_full_unstemmed Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.
title_short Oxidative Damage and Mutagenic Potency of Fast Neutron and UV-B Radiation in Pollen Mother Cells and Seed Yield of Vicia faba L.
title_sort oxidative damage and mutagenic potency of fast neutron and uv-b radiation in pollen mother cells and seed yield of vicia faba l.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771406/
https://www.ncbi.nlm.nih.gov/pubmed/24066298
http://dx.doi.org/10.1155/2013/824656
work_keys_str_mv AT abdelhaliemekram oxidativedamageandmutagenicpotencyoffastneutronanduvbradiationinpollenmothercellsandseedyieldofviciafabal
AT abdullahhanan oxidativedamageandmutagenicpotencyoffastneutronanduvbradiationinpollenmothercellsandseedyieldofviciafabal
AT alhuqailasmaa oxidativedamageandmutagenicpotencyoffastneutronanduvbradiationinpollenmothercellsandseedyieldofviciafabal