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Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy

Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self-renewal capacity, multilineage potential, and immunosuppressive property, mesenchyma...

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Autores principales: Haque, Nazmul, Rahman, Mohammad Tariqur, Abu Kasim, Noor Hayaty, Alabsi, Aied Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771429/
https://www.ncbi.nlm.nih.gov/pubmed/24068884
http://dx.doi.org/10.1155/2013/632972
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author Haque, Nazmul
Rahman, Mohammad Tariqur
Abu Kasim, Noor Hayaty
Alabsi, Aied Mohammed
author_facet Haque, Nazmul
Rahman, Mohammad Tariqur
Abu Kasim, Noor Hayaty
Alabsi, Aied Mohammed
author_sort Haque, Nazmul
collection PubMed
description Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self-renewal capacity, multilineage potential, and immunosuppressive property, mesenchymal stem cells (MSCs) are considered as an attractive source of stem cells for regenerative therapies. However, poor growth kinetics, early senescence, and genetic instability during in vitro expansion and poor engraftment after transplantation are considered to be among the major disadvantages of MSC-based regenerative therapies. A number of complex inter- and intracellular interactive signaling systems control growth, multiplication, and differentiation of MSCs in their niche. Common laboratory conditions for stem cell culture involve ambient O(2) concentration (20%) in contrast to their niche where they usually reside in 2–9% O(2). Notably, O(2) plays an important role in maintaining stem cell fate in terms of proliferation and differentiation, by regulating hypoxia-inducible factor-1 (HIF-1) mediated expression of different genes. This paper aims to describe and compare the role of normoxia (20% O(2)) and hypoxia (2–9% O(2)) on the biology of MSCs. Finally it is concluded that a hypoxic environment can greatly improve growth kinetics, genetic stability, and expression of chemokine receptors during in vitro expansion and eventually can increase efficiency of MSC-based regenerative therapies.
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spelling pubmed-37714292013-09-25 Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy Haque, Nazmul Rahman, Mohammad Tariqur Abu Kasim, Noor Hayaty Alabsi, Aied Mohammed ScientificWorldJournal Review Article Cell-based regenerative therapies, based on in vitro propagation of stem cells, offer tremendous hope to many individuals suffering from degenerative diseases that were previously deemed untreatable. Due to the self-renewal capacity, multilineage potential, and immunosuppressive property, mesenchymal stem cells (MSCs) are considered as an attractive source of stem cells for regenerative therapies. However, poor growth kinetics, early senescence, and genetic instability during in vitro expansion and poor engraftment after transplantation are considered to be among the major disadvantages of MSC-based regenerative therapies. A number of complex inter- and intracellular interactive signaling systems control growth, multiplication, and differentiation of MSCs in their niche. Common laboratory conditions for stem cell culture involve ambient O(2) concentration (20%) in contrast to their niche where they usually reside in 2–9% O(2). Notably, O(2) plays an important role in maintaining stem cell fate in terms of proliferation and differentiation, by regulating hypoxia-inducible factor-1 (HIF-1) mediated expression of different genes. This paper aims to describe and compare the role of normoxia (20% O(2)) and hypoxia (2–9% O(2)) on the biology of MSCs. Finally it is concluded that a hypoxic environment can greatly improve growth kinetics, genetic stability, and expression of chemokine receptors during in vitro expansion and eventually can increase efficiency of MSC-based regenerative therapies. Hindawi Publishing Corporation 2013-08-27 /pmc/articles/PMC3771429/ /pubmed/24068884 http://dx.doi.org/10.1155/2013/632972 Text en Copyright © 2013 Nazmul Haque et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Haque, Nazmul
Rahman, Mohammad Tariqur
Abu Kasim, Noor Hayaty
Alabsi, Aied Mohammed
Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy
title Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy
title_full Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy
title_fullStr Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy
title_full_unstemmed Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy
title_short Hypoxic Culture Conditions as a Solution for Mesenchymal Stem Cell Based Regenerative Therapy
title_sort hypoxic culture conditions as a solution for mesenchymal stem cell based regenerative therapy
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3771429/
https://www.ncbi.nlm.nih.gov/pubmed/24068884
http://dx.doi.org/10.1155/2013/632972
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